2yrf

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:2yrf.jpg|left|200px]]
[[Image:2yrf.jpg|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 2yrf |SIZE=350|CAPTION= <scene name='initialview01'>2yrf</scene>, resolution 2.70&Aring;
+
The line below this paragraph, containing "STRUCTURE_2yrf", creates the "Structure Box" on the page.
-
|SITE=
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/S-methyl-5-thioribose-1-phosphate_isomerase S-methyl-5-thioribose-1-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.23 5.3.1.23] </span>
+
or leave the SCENE parameter empty for the default display.
-
|GENE= mtnA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis])
+
-->
-
|DOMAIN=
+
{{STRUCTURE_2yrf| PDB=2yrf | SCENE= }}
-
|RELATEDENTRY=
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2yrf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yrf OCA], [http://www.ebi.ac.uk/pdbsum/2yrf PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2yrf RCSB]</span>
+
-
}}
+
'''Crystal structure of 5-methylthioribose 1-phosphate isomerase from Bacillus subtilis complexed with sulfate ion'''
'''Crystal structure of 5-methylthioribose 1-phosphate isomerase from Bacillus subtilis complexed with sulfate ion'''
Line 30: Line 27:
[[Category: Matsumura, H.]]
[[Category: Matsumura, H.]]
[[Category: Tamura, H.]]
[[Category: Tamura, H.]]
-
[[Category: crystal structure]]
+
[[Category: Crystal structure]]
-
[[Category: isomerase]]
+
[[Category: Isomerase]]
-
[[Category: methionine salvage pathway]]
+
[[Category: Methionine salvage pathway]]
-
 
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 19:15:08 2008''
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:14:40 2008''
+

Revision as of 16:15, 4 May 2008

Image:2yrf.jpg

Template:STRUCTURE 2yrf

Crystal structure of 5-methylthioribose 1-phosphate isomerase from Bacillus subtilis complexed with sulfate ion


Overview

The methionine salvage pathway (MSP) plays a crucial role in recycling a sulphahydryl derivative of the nucleoside. Recently, the genes and reactions in MSP from Bacillus subtilis have been identified, where 5-methylthioribose 1-phosphate isomerase (M1Pi) catalyzes a conversion of 5-methylthioribose 1-phosphate (MTR-1-P) to 5-methylthioribulose 1-phosphate (MTRu-1-P). Herein, we report the crystal structures of B. subtilis M1Pi (Bs-M1Pi) in complex with its product MTRu-1-P, and a sulfate at 2.4 and 2.7 A resolution, respectively. The electron density clearly shows the presence of each compound in the active site. The structural comparison with other homologous proteins explains how the substrate uptake of Bs-M1Pi may be induced by an open/closed transition of the active site. The highly conserved residues at the active site, namely, Cys160 and Asp240 are most likely to be involved in catalysis. The structural analysis sheds light on its catalytic mechanism of M1Pi.

About this Structure

2YRF is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.

Reference

Crystal structure of 5-methylthioribose 1-phosphate isomerase product complex from Bacillus subtilis: implications for catalytic mechanism., Tamura H, Saito Y, Ashida H, Inoue T, Kai Y, Yokota A, Matsumura H, Protein Sci. 2008 Jan;17(1):126-35. PMID:18156470 Page seeded by OCA on Sun May 4 19:15:08 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2yrf"
Personal tools