2yva

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[[Image:2yva.jpg|left|200px]]
[[Image:2yva.jpg|left|200px]]
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{{Structure
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|PDB= 2yva |SIZE=350|CAPTION= <scene name='initialview01'>2yva</scene>, resolution 1.85&Aring;
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The line below this paragraph, containing "STRUCTURE_2yva", creates the "Structure Box" on the page.
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|GENE= diaA(YraO) ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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{{STRUCTURE_2yva| PDB=2yva | SCENE= }}
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2yva FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yva OCA], [http://www.ebi.ac.uk/pdbsum/2yva PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2yva RCSB]</span>
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'''Crystal structure of Escherichia coli DiaA'''
'''Crystal structure of Escherichia coli DiaA'''
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[[Category: Suetsugu, M.]]
[[Category: Suetsugu, M.]]
[[Category: Yokoyama, S.]]
[[Category: Yokoyama, S.]]
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[[Category: dna binding protein]]
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[[Category: Dna binding protein]]
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[[Category: dnaa binding protein]]
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[[Category: Dnaa binding protein]]
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[[Category: intermolecular disulfide bonding]]
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[[Category: Intermolecular disulfide bonding]]
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[[Category: national project on protein structural and functional analyse]]
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[[Category: National project on protein structural and functional analyse]]
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[[Category: nppsfa]]
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[[Category: Nppsfa]]
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[[Category: putative phosphosugar binding protein]]
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[[Category: Putative phosphosugar binding protein]]
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[[Category: riken structural genomics/proteomics initiative]]
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[[Category: Riken structural genomics/proteomics initiative]]
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[[Category: rsgi]]
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[[Category: Rsgi]]
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[[Category: structural genomic]]
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[[Category: Structural genomic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 19:31:29 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:15:40 2008''
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Revision as of 16:31, 4 May 2008

Image:2yva.jpg

Template:STRUCTURE 2yva

Crystal structure of Escherichia coli DiaA


Overview

Escherichia coli DiaA is a DnaA-binding protein that is required for the timely initiation of chromosomal replication during the cell cycle. In this study, we determined the crystal structure of DiaA at 1.8 A resolution. DiaA forms a homotetramer consisting of a symmetrical pair of homodimers. Mutational analysis revealed that the DnaA-binding activity and formation of homotetramers are required for the stimulation of initiation by DiaA. DiaA tetramers can bind multiple DnaA molecules simultaneously. DiaA stimulated the assembly of multiple DnaA molecules on oriC, conformational changes in ATP-DnaA-specific initiation complexes, and unwinding of oriC duplex DNA. The mutant DiaA proteins are defective in these stimulations. DiaA associated also with ADP-DnaA, and stimulated the assembly of inactive ADP-DnaA-oriC complexes. Specific residues in the putative phosphosugar-binding motif of DiaA were required for the stimulation of initiation and formation of ATP-DnaA-specific-oriC complexes. Our data indicate that DiaA regulates initiation by a novel mechanism, in which DiaA tetramers most likely bind to multiple DnaA molecules and stimulate the assembly of specific ATP-DnaA-oriC complexes. These results suggest an essential role for DiaA in the promotion of replication initiation in a cell cycle coordinated manner.

About this Structure

2YVA is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

The interaction of DiaA and DnaA regulates the replication cycle in E. coli by directly promoting ATP DnaA-specific initiation complexes., Keyamura K, Fujikawa N, Ishida T, Ozaki S, Su'etsugu M, Fujimitsu K, Kagawa W, Yokoyama S, Kurumizaka H, Katayama T, Genes Dev. 2007 Aug 15;21(16):2083-99. PMID:17699754 Page seeded by OCA on Sun May 4 19:31:29 2008

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OCA

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