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2z8f

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[[Image:2z8f.jpg|left|200px]]
[[Image:2z8f.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 2z8f |SIZE=350|CAPTION= <scene name='initialview01'>2z8f</scene>, resolution 1.65&Aring;
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The line below this paragraph, containing "STRUCTURE_2z8f", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=AC1:Bgc+Binding+Site+For+Residue+A+4'>AC1</scene>, <scene name='pdbsite=AC2:Gal+Binding+Site+For+Residue+A+3'>AC2</scene>, <scene name='pdbsite=AC3:Nag+Binding+Site+For+Residue+A+2'>AC3</scene>, <scene name='pdbsite=AC4:Gal+Binding+Site+For+Residue+A+1'>AC4</scene>, <scene name='pdbsite=AC5:Bgc+Binding+Site+For+Residue+B+4'>AC5</scene>, <scene name='pdbsite=AC6:Gal+Binding+Site+For+Residue+B+3'>AC6</scene>, <scene name='pdbsite=AC7:Nag+Binding+Site+For+Residue+B+2'>AC7</scene>, <scene name='pdbsite=AC8:Gal+Binding+Site+For+Residue+B+1'>AC8</scene>, <scene name='pdbsite=AC9:Zn+Binding+Site+For+Residue+A+11'>AC9</scene>, <scene name='pdbsite=BC1:Zn+Binding+Site+For+Residue+B+12'>BC1</scene>, <scene name='pdbsite=BC2:Zn+Binding+Site+For+Residue+A+13'>BC2</scene>, <scene name='pdbsite=BC3:Zn+Binding+Site+For+Residue+B+14'>BC3</scene>, <scene name='pdbsite=BC4:Zn+Binding+Site+For+Residue+B+15'>BC4</scene>, <scene name='pdbsite=BC5:Na+Binding+Site+For+Residue+B+16'>BC5</scene>, <scene name='pdbsite=BC6:Na+Binding+Site+For+Residue+A+17'>BC6</scene>, <scene name='pdbsite=BC7:Na+Binding+Site+For+Residue+A+18'>BC7</scene>, <scene name='pdbsite=BC8:Mes+Binding+Site+For+Residue+A+21'>BC8</scene> and <scene name='pdbsite=BC9:Mes+Binding+Site+For+Residue+B+22'>BC9</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_2z8f| PDB=2z8f | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2z8f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2z8f OCA], [http://www.ebi.ac.uk/pdbsum/2z8f PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2z8f RCSB]</span>
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}}
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'''The galacto-N-biose-/lacto-N-biose I-binding protein (GL-BP) of the ABC transporter from Bifidobacterium longum in complex with lacto-N-tetraose'''
'''The galacto-N-biose-/lacto-N-biose I-binding protein (GL-BP) of the ABC transporter from Bifidobacterium longum in complex with lacto-N-tetraose'''
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[[Category: Suzuki, R.]]
[[Category: Suzuki, R.]]
[[Category: Wada, J.]]
[[Category: Wada, J.]]
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[[Category: abc transporter]]
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[[Category: Abc transporter]]
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[[Category: human milk oligosacchalide]]
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[[Category: Human milk oligosacchalide]]
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[[Category: mucin core-1]]
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[[Category: Mucin core-1]]
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[[Category: sugar binding protein]]
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[[Category: Sugar binding protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 20:02:24 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:19:22 2008''
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Revision as of 17:02, 4 May 2008

Image:2z8f.jpg

Template:STRUCTURE 2z8f

The galacto-N-biose-/lacto-N-biose I-binding protein (GL-BP) of the ABC transporter from Bifidobacterium longum in complex with lacto-N-tetraose


Overview

A recombinant galacto-N-biose-/lacto-N-biose I-binding protein (GL-BP) from Bifidobacterium longum JCM1217 has been prepared and crystallized by the hanging-drop vapour-diffusion method using 10 mg ml(-1) purified enzyme, 0.01 M zinc sulfate, 0.1 M MES buffer pH 5.9-6.4 and 20-22%(v/v) PEG MME 550 in the presence of 5 mM disaccharide ligands. Suitable crystals grew after 10 d incubation at 293 K. The crystals belong to space group C222(1), with unit-cell parameters a = 106.3, b = 143.6, c = 114.6 A for the lacto-N-biose I complex and a = 106.4, b = 143.4, c = 115.5 A for the galacto-N-biose complex, and diffracted to 1.85 and 1.99 A resolution, respectively.

About this Structure

2Z8F is a Single protein structure of sequence from Bifidobacterium longum. Full crystallographic information is available from OCA.

Reference

Purification, crystallization and preliminary X-ray analysis of the galacto-N-biose-/lacto-N-biose I-binding protein (GL-BP) of the ABC transporter from Bifidobacterium longum JCM1217., Wada J, Suzuki R, Fushinobu S, Kitaoka M, Wakagi T, Shoun H, Ashida H, Kumagai H, Katayama T, Yamamoto K, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Sep 1;63(Pt, 9):751-3. Epub 2007 Aug 10. PMID:17768345 Page seeded by OCA on Sun May 4 20:02:24 2008

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