2z8x

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[[Image:2z8x.gif|left|200px]]
[[Image:2z8x.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2z8x |SIZE=350|CAPTION= <scene name='initialview01'>2z8x</scene>, resolution 1.48&Aring;
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The line below this paragraph, containing "STRUCTURE_2z8x", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_2z8x| PDB=2z8x | SCENE= }}
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|RELATEDENTRY=[[2z8z|2Z8Z]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2z8x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2z8x OCA], [http://www.ebi.ac.uk/pdbsum/2z8x PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2z8x RCSB]</span>
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}}
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'''Crystal structure of extracellular lipase from Pseudomonas sp. MIS38'''
'''Crystal structure of extracellular lipase from Pseudomonas sp. MIS38'''
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[[Category: Takano, K.]]
[[Category: Takano, K.]]
[[Category: You, D J.]]
[[Category: You, D J.]]
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[[Category: beta roll]]
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[[Category: Beta roll]]
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[[Category: calcium binding protein]]
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[[Category: Calcium binding protein]]
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[[Category: family i 3 lipase]]
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[[Category: Family i 3 lipase]]
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[[Category: hydrolase]]
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[[Category: Hydrolase]]
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[[Category: rtx protein]]
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[[Category: Rtx protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 20:03:10 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:19:29 2008''
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Revision as of 17:03, 4 May 2008

Image:2z8x.gif

Template:STRUCTURE 2z8x

Crystal structure of extracellular lipase from Pseudomonas sp. MIS38


Overview

The crystal structure of a family I.3 lipase from Pseudomonas sp. MIS38 in a closed conformation was determined at 1.5A resolution. This structure highly resembles that of Serratia marcescens LipA in an open conformation, except for the structures of two lids. Lid1 is anchored by a Ca2+ ion (Ca1) in an open conformation, but lacks this Ca1 site and greatly changes its structure and position in a closed conformation. Lid2 forms a helical hairpin in an open conformation, but does not form it and covers the active site in a closed conformation. Based on these results, we discuss on the lid-opening mechanism.

About this Structure

2Z8X is a Single protein structure of sequence from Pseudomonas sp. mis38. Full crystallographic information is available from OCA.

Reference

Crystal structure of a family I.3 lipase from Pseudomonas sp. MIS38 in a closed conformation., Angkawidjaja C, You DJ, Matsumura H, Kuwahara K, Koga Y, Takano K, Kanaya S, FEBS Lett. 2007 Oct 30;581(26):5060-4. Epub 2007 Oct 1. PMID:17923123 Page seeded by OCA on Sun May 4 20:03:10 2008

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