2za6
From Proteopedia
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'''recombinant horse L-chain apoferritin''' | '''recombinant horse L-chain apoferritin''' | ||
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[[Category: Tame, J R.H.]] | [[Category: Tame, J R.H.]] | ||
[[Category: Yamashita, I.]] | [[Category: Yamashita, I.]] | ||
| - | [[Category: | + | [[Category: Acetylation]] |
| - | [[Category: | + | [[Category: Ferric iron binding]] |
| - | [[Category: | + | [[Category: Iron storage]] |
| - | [[Category: | + | [[Category: Metal binding protein]] |
| - | [[Category: | + | [[Category: Metal-binding]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 20:05:17 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 17:05, 4 May 2008
recombinant horse L-chain apoferritin
Overview
The denaturation of recombinant horse L-chain apoferritin (rLF), which is composed of 24 L-chain subunits, in acidic solution was studied. Using two rLF mutants, lacking four (Fer4) or eight (Fer8) N-terminal amino acid residues, the effect of N-terminal residues on the protein's stability was investigated. Of the two mutants and wild-type rLF, the tertiary and secondary structures of Fer8 were found to be most sensitive to an acidic environment. The Fer8 protein dissociated easily into subunit dimers at or below pH 2.0. Comparing the crystal structures of the mutant proteins, deletion of the N-terminal residues was found to result in fewer inter- and intra-subunit hydrogen bonds. The loss of these bonds is assumed to be responsible for lower endurance against acidic denaturation in N-terminus-deleted mutants. These results indicated that the inter- and intra-subunit hydrogen bonds of N-terminal residues affect the denaturation, especially oligomer formation of apoferritin subunits and will be of use in designing ferritin-based nanodevices.
About this Structure
2ZA6 is a Single protein structure of sequence from Equus caballus. Full crystallographic information is available from OCA.
Reference
Effect of N-terminal Residues on the Structural Stability of Recombinant Horse L-chain Apoferritin in an Acidic Environment., Yoshizawa K, Mishima Y, Park SY, Heddle JG, Tame JR, Iwahori K, Kobayashi M, Yamashita I, J Biochem. 2007 Dec;142(6):707-713. Epub 2007 Oct 15. PMID:17938140 Page seeded by OCA on Sun May 4 20:05:17 2008
