3b3r

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[[Image:3b3r.jpg|left|200px]]
[[Image:3b3r.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 3b3r |SIZE=350|CAPTION= <scene name='initialview01'>3b3r</scene>, resolution 0.980&Aring;
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The line below this paragraph, containing "STRUCTURE_3b3r", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=FAE:FLAVIN-N7+PROTONATED-ADENINE+DINUCLEOTIDE'>FAE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Cholesterol_oxidase Cholesterol oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.3.6 1.1.3.6] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_3b3r| PDB=3b3r | SCENE= }}
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|RELATEDENTRY=[[1mxt|1MXT]], [[3b6d|3B6D]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3b3r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b3r OCA], [http://www.ebi.ac.uk/pdbsum/3b3r PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=3b3r RCSB]</span>
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}}
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'''Crystal structure of Streptomyces cholesterol oxidase H447Q/E361Q mutant bound to glycerol (0.98A)'''
'''Crystal structure of Streptomyces cholesterol oxidase H447Q/E361Q mutant bound to glycerol (0.98A)'''
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[[Category: Tang, H.]]
[[Category: Tang, H.]]
[[Category: Vrielink, A.]]
[[Category: Vrielink, A.]]
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[[Category: cholesterol metabolism]]
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[[Category: Cholesterol metabolism]]
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[[Category: cholesterol oxidase]]
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[[Category: Cholesterol oxidase]]
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[[Category: covalently-modified flavin]]
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[[Category: Covalently-modified flavin]]
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[[Category: fad]]
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[[Category: Fad]]
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[[Category: flavin]]
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[[Category: Flavin]]
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[[Category: flavoenzyme]]
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[[Category: Flavoenzyme]]
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[[Category: flavoprotein]]
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[[Category: Flavoprotein]]
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[[Category: lipid metabolism]]
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[[Category: Lipid metabolism]]
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[[Category: oxidoreductase]]
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[[Category: Oxidoreductase]]
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[[Category: secreted]]
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[[Category: Secreted]]
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[[Category: steroid metabolism]]
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[[Category: Steroid metabolism]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 20:21:45 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:22:48 2008''
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Revision as of 17:21, 4 May 2008

Image:3b3r.jpg

Template:STRUCTURE 3b3r

Crystal structure of Streptomyces cholesterol oxidase H447Q/E361Q mutant bound to glycerol (0.98A)


Overview

Two high-resolution structures of a double mutant of bacterial cholesterol oxidase in the presence or absence of a ligand, glycerol, are presented, showing the trajectory of glycerol as it binds in a Michaelis complex-like position in the active site. A group of three aromatic residues forces the oxidized isoalloxazine moiety to bend along the N5-N10 axis as a response to the binding of glycerol in the active site. Movement of these aromatic residues is only observed in the glycerol-bound structure, indicating that some tuning of the FAD redox potential is caused by the formation of the Michaelis complex during regular catalysis. This structural study suggests a possible mechanism of substrate-assisted flavin activation, improves our understanding of the interplay between the enzyme, its flavin cofactor and its substrate, and is of use to the future design of effective cholesterol oxidase inhibitors.

About this Structure

3B3R is a Single protein structure of sequence from Streptomyces sp.. Full crystallographic information is available from OCA.

Reference

Distortion of flavin geometry is linked to ligand binding in cholesterol oxidase., Lyubimov AY, Heard K, Tang H, Sampson NS, Vrielink A, Protein Sci. 2007 Dec;16(12):2647-56. PMID:18029419 Page seeded by OCA on Sun May 4 20:21:45 2008

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