3b4b

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[[Image:3b4b.gif|left|200px]]
[[Image:3b4b.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 3b4b |SIZE=350|CAPTION= <scene name='initialview01'>3b4b</scene>, resolution 2.7&Aring;
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The line below this paragraph, containing "STRUCTURE_3b4b", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=3AD:3&#39;-DEOXYADENOSINE'>3AD</scene>, <scene name='pdbligand=A:ADENOSINE-5&#39;-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5&#39;-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5&#39;-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=GLP:GLUCOSAMINE+6-PHOSPHATE'>GLP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=U:URIDINE-5&#39;-MONOPHOSPHATE'>U</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE=
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|DOMAIN=
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{{STRUCTURE_3b4b| PDB=3b4b | SCENE= }}
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|RELATEDENTRY=[[3b4a|3B4A]], [[3b4c|3B4C]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3b4b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b4b OCA], [http://www.ebi.ac.uk/pdbsum/3b4b PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=3b4b RCSB]</span>
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'''T. tengcongensis glmS ribozyme with G40A mutation, bound to glucosamine-6-phosphate and a substrate RNA with a 2'5'-phosphodiester linkage'''
'''T. tengcongensis glmS ribozyme with G40A mutation, bound to glucosamine-6-phosphate and a substrate RNA with a 2'5'-phosphodiester linkage'''
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[[Category: Amare, A R.Ferre-D.]]
[[Category: Amare, A R.Ferre-D.]]
[[Category: Klein, D J.]]
[[Category: Klein, D J.]]
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[[Category: glucosamine-6-phosphate]]
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[[Category: Glucosamine-6-phosphate]]
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[[Category: riboswitch]]
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[[Category: Riboswitch]]
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[[Category: ribozyme]]
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[[Category: Ribozyme]]
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[[Category: rna]]
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[[Category: Rna]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 20:22:50 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:22:54 2008''
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Revision as of 17:22, 4 May 2008

Image:3b4b.gif

Template:STRUCTURE 3b4b

T. tengcongensis glmS ribozyme with G40A mutation, bound to glucosamine-6-phosphate and a substrate RNA with a 2'5'-phosphodiester linkage


Overview

The glmS ribozyme is a catalytic riboswitch that is activated for endonucleolytic cleavage by the coenzyme glucosamine-6-phosphate. Using kinetic assays and X-ray crystallography, we identify an active-site mutation of a conserved guanine that abolishes catalysis without perturbing coenzyme binding. Our results provide evidence that coenzyme function requires a specific nucleobase to interact with the nucleophile of the cleavage reaction.

About this Structure

3B4B is a Protein complex structure of sequences from Thermoanaerobacter tengcongensis. Full crystallographic information is available from OCA.

Reference

Essential role of an active-site guanine in glmS ribozyme catalysis., Klein DJ, Been MD, Ferre-D'Amare AR, J Am Chem Soc. 2007 Dec 5;129(48):14858-9. Epub 2007 Nov 9. PMID:17990888 Page seeded by OCA on Sun May 4 20:22:50 2008

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