1akk
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(New page: 200px<br /><applet load="1akk" size="450" color="white" frame="true" align="right" spinBox="true" caption="1akk" /> '''SOLUTION STRUCTURE OF OXIDIZED HORSE HEART C...)
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Revision as of 08:48, 20 November 2007
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SOLUTION STRUCTURE OF OXIDIZED HORSE HEART CYTOCHROME C, NMR, MINIMIZED AVERAGE STRUCTURE
Overview
The solution structure of oxidized horse heart cytochrome c was obtained, at pH 7.0 in 100 mM phosphate buffer from 2278 NOEs and 241 pseudocontact, shift constraints. The final structure was refined through restrained, energy minimization. A 35-member family, with RMSD values with respect to, the average structure of 0.70 +/- 0.11 A and 1.21 +/- 0.14 A for the, backbone and all heavy atoms, respectively, and with an average penalty, function of 130 +/- 4.0 kJ/mol and 84 +/- 3.7 kJ/mol for NOE and, pseudocontact shift constraints, respectively (corresponding to a target, function of 0.9 A2 and 0.2 A2), was obtained. The solution structure is, somewhat different from that recently reported (Qi et al., 1996) and, appears to be similar to the X-ray structure of the same oxidation state, (Bushnell et al., 1990). A noticeable difference is a rotation of 17 +/- 8, degrees of the imidazole plane between solid and solution structure., Detailed and accurate structural determinations are important within the, frame of the current debate of the structural rearrangements occurring, upon oxidation or reduction. From the obtained magnetic susceptibility, tensor a separation of the hyperfine shifts into their contact and, pseudocontact contributions is derived and compared to that of the, analogous isoenzyme from S. cerevisiae and to previous results.
About this Structure
1AKK is a Single protein structure of sequence from Equus caballus with HEC as ligand. Full crystallographic information is available from OCA.
Reference
Solution structure of oxidized horse heart cytochrome c., Banci L, Bertini I, Gray HB, Luchinat C, Reddig T, Rosato A, Turano P, Biochemistry. 1997 Aug 12;36(32):9867-77. PMID:9245419
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