3b8a
From Proteopedia
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'''Crystal structure of yeast hexokinase PI in complex with glucose''' | '''Crystal structure of yeast hexokinase PI in complex with glucose''' | ||
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[[Category: Kuser, P.]] | [[Category: Kuser, P.]] | ||
[[Category: Polikarpov, I.]] | [[Category: Polikarpov, I.]] | ||
| - | [[Category: | + | [[Category: Allosteric enzyme]] |
| - | [[Category: | + | [[Category: Atp-binding]] |
| - | [[Category: | + | [[Category: Glycolysis]] |
| - | [[Category: | + | [[Category: Induced fit]] |
| - | [[Category: | + | [[Category: Kinase]] |
| - | [[Category: | + | [[Category: Nucleotide-binding]] |
| - | [[Category: | + | [[Category: Phosphorylation]] |
| - | [[Category: | + | [[Category: Transferase]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 20:30:30 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 17:30, 4 May 2008
Crystal structure of yeast hexokinase PI in complex with glucose
Overview
Hexokinase is the first enzyme in the glycolytic pathway that catalyzes the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. Two yeast hexokinase isozymes are known, namely PI and PII. Here we redetermined the crystal structure of yeast hexokinase PI from Saccharomyces cerevisiae as a complex with its substrate, glucose, and refined it at 2.95 A resolution. Comparison of the holo-PI yeast hexokinase and apo-hexokinase structures shows in detail the rigid body domain closure and specific loop movements as glucose binds and sheds more light on structural basis of the "induced fit" mechanism of reaction in the HK enzymatic action. We also performed statistical coupling analysis of the hexokinase family, which reveals two co-evolved continuous clusters of amino acid residues and shows that the evolutionary coupled amino acid residues are mostly confined to the active site and the hinge region, further supporting the importance of these parts of the protein for the enzymatic catalysis. Proteins 2008. (c) 2008 Wiley-Liss, Inc.
About this Structure
3B8A is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Crystal structure of yeast hexokinase PI in complex with glucose: A classical "induced fit" example revised., Kuser P, Cupri F, Bleicher L, Polikarpov I, Proteins. 2008 Feb 7;. PMID:18260108 Page seeded by OCA on Sun May 4 20:30:30 2008
