3bgw
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:3bgw.jpg|left|200px]] | [[Image:3bgw.jpg|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_3bgw", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | + | --> | |
| - | + | {{STRUCTURE_3bgw| PDB=3bgw | SCENE= }} | |
| - | + | ||
| - | | | + | |
| - | }} | + | |
'''The Structure Of A DnaB-Like Replicative Helicase And Its Interactions With Primase''' | '''The Structure Of A DnaB-Like Replicative Helicase And Its Interactions With Primase''' | ||
| Line 31: | Line 28: | ||
[[Category: Wang, G.]] | [[Category: Wang, G.]] | ||
[[Category: Zhang, Y.]] | [[Category: Zhang, Y.]] | ||
| - | [[Category: | + | [[Category: Atpase]] |
| - | [[Category: | + | [[Category: Replication]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 20:45:01 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 17:45, 4 May 2008
The Structure Of A DnaB-Like Replicative Helicase And Its Interactions With Primase
Overview
Helicases are essential enzymes for DNA replication, a fundamental process in all living organisms. The DnaB family are hexameric replicative helicases that unwind duplex DNA and coordinate with RNA primase and other proteins at the replication fork in prokaryotes. Here, we report the full-length crystal structure of G40P, a DnaB family helicase. The hexamer complex reveals an unusual architectural feature and a new type of assembly mechanism. The hexamer has two tiers: a three-fold symmetric N-terminal tier and a six-fold symmetric C-terminal tier. Monomers with two different conformations, termed cis and trans, come together to provide a topological solution for the dual symmetry within a hexamer. Structure-guided mutational studies indicate an important role for the N-terminal tier in binding primase and regulating primase-mediated stimulation of helicase activity. This study provides insights into the structural and functional interplay between G40P helicase and DnaG primase.
About this Structure
3BGW is a Single protein structure of sequence from Bacillus phage spp1. Full crystallographic information is available from OCA.
Reference
The structure of a DnaB-family replicative helicase and its interactions with primase., Wang G, Klein MG, Tokonzaba E, Zhang Y, Holden LG, Chen XS, Nat Struct Mol Biol. 2008 Jan;15(1):94-100. Epub 2007 Dec 23. PMID:18157148 Page seeded by OCA on Sun May 4 20:45:01 2008
