3bk1
From Proteopedia
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'''Crystal Structure Analysis of RNase J''' | '''Crystal Structure Analysis of RNase J''' | ||
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[[Category: Zig, L.]] | [[Category: Zig, L.]] | ||
[[Category: 5'-3' exoribonuclease]] | [[Category: 5'-3' exoribonuclease]] | ||
| - | [[Category: | + | [[Category: Endoribonuclease]] |
| - | [[Category: | + | [[Category: Metal dependent hydrolase]] |
| - | [[Category: | + | [[Category: Metallo-beta-lactamase]] |
| - | [[Category: | + | [[Category: Rnase j]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 20:52:09 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 17:52, 4 May 2008
Crystal Structure Analysis of RNase J
Overview
The maturation and stability of RNA transcripts is controlled by a combination of endo- and exoRNases. RNase J is unique, as it combines an RNase E-like endoribonucleolytic and a 5'-to-3' exoribonucleolytic activity in a single polypeptide. The structural basis for this dual activity is unknown. Here we report the crystal structures of Thermus thermophilus RNase J and its complex with uridine 5'-monophosphate. A binding pocket coordinating the phosphate and base moieties of the nucleotide in the vicinity of the catalytic center provide a rationale for the 5'-monophosphate-dependent 5'-to-3' exoribonucleolytic activity. We show that this dependence is strict; an initial 5'-PPP transcript cannot be degraded exonucleolytically from the 5'-end. Our results suggest that RNase J might switch promptly from endo- to exonucleolytic mode on the same RNA, a property that has important implications for RNA metabolism in numerous prokaryotic organisms and plant organelles containing RNase J orthologs.
About this Structure
3BK1 is a Single protein structure of sequence from Thermus thermophilus. Full crystallographic information is available from OCA.
Reference
Structural insights into the dual activity of RNase J., de la Sierra-Gallay IL, Zig L, Jamalli A, Putzer H, Nat Struct Mol Biol. 2008 Feb;15(2):206-12. Epub 2008 Jan 20. PMID:18204464 Page seeded by OCA on Sun May 4 20:52:09 2008
