3bk2

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[[Image:3bk2.jpg|left|200px]]
[[Image:3bk2.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 3bk2 |SIZE=350|CAPTION= <scene name='initialview01'>3bk2</scene>, resolution 2.100&Aring;
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The line below this paragraph, containing "STRUCTURE_3bk2", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=AC1:So4+Binding+Site+For+Residue+A+947'>AC1</scene>, <scene name='pdbsite=AC2:Zn+Binding+Site+For+Residue+A+948'>AC2</scene>, <scene name='pdbsite=AC3:Zn+Binding+Site+For+Residue+A+949'>AC3</scene>, <scene name='pdbsite=AC4:U5p+Binding+Site+For+Residue+A+950'>AC4</scene>, <scene name='pdbsite=AC5:Gol+Binding+Site+For+Residue+A+601'>AC5</scene>, <scene name='pdbsite=AC6:Gol+Binding+Site+For+Residue+A+602'>AC6</scene> and <scene name='pdbsite=AC7:Gol+Binding+Site+For+Residue+A+603'>AC7</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=U5P:URIDINE-5&#39;-MONOPHOSPHATE'>U5P</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE= TTC0775 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=274 Thermus thermophilus])
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-->
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|DOMAIN=
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{{STRUCTURE_3bk2| PDB=3bk2 | SCENE= }}
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|RELATEDENTRY=[[3bk1|3BK1]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bk2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bk2 OCA], [http://www.ebi.ac.uk/pdbsum/3bk2 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=3bk2 RCSB]</span>
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}}
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'''Crystal Structure Analysis of the RNase J/UMP complex'''
'''Crystal Structure Analysis of the RNase J/UMP complex'''
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[[Category: Sierra-Gallay, I L.de la.]]
[[Category: Sierra-Gallay, I L.de la.]]
[[Category: Zig, L.]]
[[Category: Zig, L.]]
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[[Category: endoribonuclease]]
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[[Category: Endoribonuclease]]
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[[Category: exoribonuclease]]
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[[Category: Exoribonuclease]]
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[[Category: metal dependent hydrolase]]
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[[Category: Metal dependent hydrolase]]
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[[Category: metallo-beta-lactamase]]
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[[Category: Metallo-beta-lactamase]]
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[[Category: rnase j]]
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[[Category: Rnase j]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 20:52:15 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:27:07 2008''
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Revision as of 17:52, 4 May 2008

Image:3bk2.jpg

Template:STRUCTURE 3bk2

Crystal Structure Analysis of the RNase J/UMP complex


Overview

The maturation and stability of RNA transcripts is controlled by a combination of endo- and exoRNases. RNase J is unique, as it combines an RNase E-like endoribonucleolytic and a 5'-to-3' exoribonucleolytic activity in a single polypeptide. The structural basis for this dual activity is unknown. Here we report the crystal structures of Thermus thermophilus RNase J and its complex with uridine 5'-monophosphate. A binding pocket coordinating the phosphate and base moieties of the nucleotide in the vicinity of the catalytic center provide a rationale for the 5'-monophosphate-dependent 5'-to-3' exoribonucleolytic activity. We show that this dependence is strict; an initial 5'-PPP transcript cannot be degraded exonucleolytically from the 5'-end. Our results suggest that RNase J might switch promptly from endo- to exonucleolytic mode on the same RNA, a property that has important implications for RNA metabolism in numerous prokaryotic organisms and plant organelles containing RNase J orthologs.

About this Structure

3BK2 is a Single protein structure of sequence from Thermus thermophilus. Full crystallographic information is available from OCA.

Reference

Structural insights into the dual activity of RNase J., de la Sierra-Gallay IL, Zig L, Jamalli A, Putzer H, Nat Struct Mol Biol. 2008 Feb;15(2):206-12. Epub 2008 Jan 20. PMID:18204464 Page seeded by OCA on Sun May 4 20:52:15 2008

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