3boy
From Proteopedia
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'''Crystal structure of the HutP antitermination complex bound to the HUT mRNA''' | '''Crystal structure of the HutP antitermination complex bound to the HUT mRNA''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | 3BOY is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. This structure supersedes the now removed PDB entry | + | 3BOY is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2gzt 2gzt]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BOY OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Shinkai, A.]] | [[Category: Shinkai, A.]] | ||
[[Category: Yokoyama, S.]] | [[Category: Yokoyama, S.]] | ||
| - | [[Category: | + | [[Category: Activator]] |
| - | [[Category: | + | [[Category: Anti-termination]] |
| - | [[Category: | + | [[Category: Histidine metabolism]] |
| - | [[Category: | + | [[Category: Hutp]] |
| - | [[Category: | + | [[Category: Hutp-rna complex]] |
| - | [[Category: | + | [[Category: National project on protein structural and functional analyse]] |
| - | [[Category: | + | [[Category: Nppsfa]] |
| - | [[Category: | + | [[Category: Riken structural genomics/proteomics initiative]] |
| - | [[Category: | + | [[Category: Rna-binding]] |
| - | [[Category: | + | [[Category: Rsgi]] |
| - | [[Category: | + | [[Category: Structural genomic]] |
| - | [[Category: | + | [[Category: Transcription regulation]] |
| - | [[Category: | + | [[Category: Transcription/rna complex]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 20:58:12 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 17:58, 4 May 2008
Crystal structure of the HutP antitermination complex bound to the HUT mRNA
Overview
HutP is an L-histidine-activated RNA binding protein that regulates the expression of the histidine utilization (hut) operon in Bacillus subtilis by binding to cis-acting regulatory sequences on the hut mRNA. The crystal structure of HutP complexed with an L-histidine analog showed a novel fold; there are four antiparallel beta strands in the central region of each monomer, with two alpha helices each on the front and back. Two HutP monomers form a dimer, and three dimers are arranged in crystallographic 3-fold symmetry to form a hexamer. A histidine analog was located in between the two monomers of HutP, with the imidazole group of L-histidine hydrogen bonded to Glu81. An activation mechanism is proposed based on the identification of key residues of HutP. The HutP binding region in hut mRNA was defined: it consists of three UAG trinucleotide motifs separated by four spacer nucleotides. Residues of HutP potentially important for RNA binding were identified.
About this Structure
3BOY is a Protein complex structure of sequences from Bacillus subtilis. This structure supersedes the now removed PDB entry 2gzt. Full crystallographic information is available from OCA.
Reference
Crystal structure of activated HutP; an RNA binding protein that regulates transcription of the hut operon in Bacillus subtilis., Kumarevel T, Fujimoto Z, Karthe P, Oda M, Mizuno H, Kumar PK, Structure. 2004 Jul;12(7):1269-80. PMID:15242603 Page seeded by OCA on Sun May 4 20:58:12 2008
Categories: Bacillus subtilis | Protein complex | Balasundaresan, D. | Jeyakanthan, J. | Kumar, P K.R. | Kumarevel, T S. | RSGI, RIKEN Structural Genomics/Proteomics Initiative. | Shinkai, A. | Yokoyama, S. | Activator | Anti-termination | Histidine metabolism | Hutp | Hutp-rna complex | National project on protein structural and functional analyse | Nppsfa | Riken structural genomics/proteomics initiative | Rna-binding | Rsgi | Structural genomic | Transcription regulation | Transcription/rna complex
