3bqa

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[[Image:3bqa.jpg|left|200px]]
[[Image:3bqa.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 3bqa |SIZE=350|CAPTION= <scene name='initialview01'>3bqa</scene>, resolution 2.000&Aring;
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The line below this paragraph, containing "STRUCTURE_3bqa", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=AC1:So4+Binding+Site+For+Residue+A+1'>AC1</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidine_kinase Histidine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.13.3 2.7.13.3] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= phoQ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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-->
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|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=pfam08918 PhoQ_Sensor]</span>
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{{STRUCTURE_3bqa| PDB=3bqa | SCENE= }}
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|RELATEDENTRY=[[3bq8|3BQ8]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bqa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bqa OCA], [http://www.ebi.ac.uk/pdbsum/3bqa PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=3bqa RCSB]</span>
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}}
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'''Crystal Structure of an E.coli PhoQ Sensor Domain Mutant'''
'''Crystal Structure of an E.coli PhoQ Sensor Domain Mutant'''
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[[Category: Hendrickson, W A.]]
[[Category: Hendrickson, W A.]]
[[Category: Waldburger, C D.]]
[[Category: Waldburger, C D.]]
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[[Category: atp-binding]]
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[[Category: Atp-binding]]
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[[Category: histidine kinase sensor domain]]
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[[Category: Histidine kinase sensor domain]]
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[[Category: inner membrane]]
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[[Category: Inner membrane]]
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[[Category: magnesium]]
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[[Category: Magnesium]]
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[[Category: membrane]]
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[[Category: Membrane]]
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[[Category: metal-binding]]
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[[Category: Metal-binding]]
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[[Category: nucleotide-binding]]
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[[Category: Nucleotide-binding]]
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[[Category: phosphoprotein]]
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[[Category: Phosphoprotein]]
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[[Category: signaling protein]]
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[[Category: Signaling protein]]
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[[Category: transferase]]
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[[Category: Transferase]]
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[[Category: transmembrane]]
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[[Category: Transmembrane]]
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[[Category: two-component regulatory system]]
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[[Category: Two-component regulatory system]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 21:00:29 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 2 11:32:44 2008''
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Revision as of 18:00, 4 May 2008

Template:STRUCTURE 3bqa

Crystal Structure of an E.coli PhoQ Sensor Domain Mutant


Overview

The PhoP-PhoQ two-component system is a well studied bacterial signaling system that regulates virulence and stress response. Catalytic activity of the histidine kinase sensor protein PhoQ is activated by low extracellular concentrations of divalent cations such as Mg2+, and subsequently the response regulator PhoP is activated in turn through a classic phosphotransfer pathway that is typical in such systems. The PhoQ sensor domains of enteric bacteria contain an acidic cluster of residues (EDDDDAE) that has been implicated in direct binding to divalent cations. We have determined crystal structures of the wild-type Escherichia coli PhoQ periplasmic sensor domain and of a mutant variant in which the acidic cluster was neutralized to conservative uncharged residues (QNNNNAQ). The PhoQ domain structure is similar to that of DcuS and CitA sensor domains, and this PhoQ-DcuS-CitA (PDC) sensor fold is seen to be distinct from the superficially similar PAS domain fold. Analysis of the wild-type structure reveals a dimer that allows for the formation of a salt bridge across the dimer interface between Arg50' and Asp179 and with nickel ions bound to aspartate residues in the acidic cluster. The physiological importance of the salt bridge to in vivo PhoQ function has been confirmed by mutagenesis. The mutant structure has an alternative, non-physiological dimeric association.

About this Structure

3BQA is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Crystal structure of a functional simer of the PhoQ sensor domain., Cheung J, Bingman CA, Reyngold M, Hendrickson WA, Waldburger CD, J Biol Chem. 2008 Mar 18;. PMID:18348979 Page seeded by OCA on Sun May 4 21:00:29 2008

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