3bsn

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[[Image:3bsn.jpg|left|200px]]
[[Image:3bsn.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 3bsn |SIZE=350|CAPTION= <scene name='initialview01'>3bsn</scene>, resolution 1.80&Aring;
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The line below this paragraph, containing "STRUCTURE_3bsn", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=AC1:Mn+Binding+Site+For+Residue+A+511'>AC1</scene>, <scene name='pdbsite=AC2:Mn+Binding+Site+For+Residue+A+512'>AC2</scene>, <scene name='pdbsite=AC3:Mn+Binding+Site+For+Residue+A+513'>AC3</scene>, <scene name='pdbsite=AC4:N5c+Binding+Site+For+Residue+A+514'>AC4</scene>, <scene name='pdbsite=AC5:Gol+Binding+Site+For+Residue+A+515'>AC5</scene>, <scene name='pdbsite=AC6:Gol+Binding+Site+For+Residue+P+9'>AC6</scene>, <scene name='pdbsite=AC7:Gol+Binding+Site+For+Residue+A+516'>AC7</scene> and <scene name='pdbsite=AC8:Gol+Binding+Site+For+Residue+T+10'>AC8</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=C:CYTIDINE-5&#39;-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5&#39;-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=N5C:5-NITROCYTIDINE+5&#39;-(TETRAHYDROGEN+TRIPHOSPHATE)'>N5C</scene>, <scene name='pdbligand=N5M:5-NITROCYTIDINE+5&#39;-(DIHYDROGEN+PHOSPHATE)'>N5M</scene>, <scene name='pdbligand=U:URIDINE-5&#39;-MONOPHOSPHATE'>U</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA-directed_RNA_polymerase RNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.48 2.7.7.48] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=cd01699 RNA_dep_RNAP]</span>
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{{STRUCTURE_3bsn| PDB=3bsn | SCENE= }}
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|RELATEDENTRY=[[1sh0|1SH0]], [[3bso|3BSO]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bsn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bsn OCA], [http://www.ebi.ac.uk/pdbsum/3bsn PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=3bsn RCSB]</span>
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}}
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'''Norwalk Virus polymerase bound to 5-nitrocytidine triphosphate and primer-template RNA'''
'''Norwalk Virus polymerase bound to 5-nitrocytidine triphosphate and primer-template RNA'''
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[[Category: Ng, K K.S.]]
[[Category: Ng, K K.S.]]
[[Category: Zamyatkin, D F.]]
[[Category: Zamyatkin, D F.]]
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[[Category: antiviral enzyme inhibitor]]
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[[Category: Antiviral enzyme inhibitor]]
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[[Category: helicase]]
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[[Category: Helicase]]
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[[Category: hydrolase]]
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[[Category: Hydrolase]]
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[[Category: nucleotide-binding]]
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[[Category: Nucleotide-binding]]
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[[Category: nucleotidyltransferase]]
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[[Category: Nucleotidyltransferase]]
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[[Category: rna replication]]
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[[Category: Rna replication]]
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[[Category: rna-dependent rna polymerase]]
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[[Category: Rna-dependent rna polymerase]]
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[[Category: rna-directed rna polymerase]]
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[[Category: Rna-directed rna polymerase]]
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[[Category: transferase]]
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[[Category: Transferase]]
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[[Category: transferase/rna complex]]
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[[Category: Transferase/rna complex]]
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[[Category: viral replication]]
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[[Category: Viral replication]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 21:03:58 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 2 11:32:43 2008''
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Revision as of 18:03, 4 May 2008

Image:3bsn.jpg

Template:STRUCTURE 3bsn

Norwalk Virus polymerase bound to 5-nitrocytidine triphosphate and primer-template RNA


Overview

Crystal structures of Norwalk virus polymerase bound to an RNA primer-template duplex and either the natural substrate CTP or the inhibitor 5-nitrocytidine triphosphate have been determined to 1.8A resolution. These structures reveal a closed conformation of the polymerase that differs significantly from previously determined open structures of calicivirus and picornavirus polymerases. These closed complexes are trapped immediately prior to the nucleotidyl transfer reaction, with the triphosphate group of the nucleotide bound to two manganese ions at the active site, poised for reaction to the 3'-hydroxyl group of the RNA primer. The positioning of the 5-nitrocytidine triphosphate nitro group between the alpha-phosphate and the 3'-hydroxyl group of the primer suggests a novel, general approach for the design of antiviral compounds mimicking natural nucleosides and nucleotides.

About this Structure

3BSN is a Protein complex structure of sequences from Norwalk virus. Full crystallographic information is available from OCA.

Reference

Structural insights into mechanisms of catalysis and inhibition in norwalk virus polymerase., Zamyatkin DF, Parra F, Alonso JM, Harki DA, Peterson BR, Grochulski P, Ng KK, J Biol Chem. 2008 Mar 21;283(12):7705-12. Epub 2008 Jan 9. PMID:18184655 Page seeded by OCA on Sun May 4 21:03:58 2008

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OCA

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