3bts

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[[Image:3bts.jpg|left|200px]]
[[Image:3bts.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 3bts |SIZE=350|CAPTION= <scene name='initialview01'>3bts</scene>, resolution 2.70&Aring;
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The line below this paragraph, containing "STRUCTURE_3bts", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=AC1:Nad+Binding+Site+For+Residue+A+436'>AC1</scene> and <scene name='pdbsite=AC2:Nad+Binding+Site+For+Residue+B+436'>AC2</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE= GAL80 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])
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-->
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|DOMAIN=
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{{STRUCTURE_3bts| PDB=3bts | SCENE= }}
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|RELATEDENTRY=[[3btu|3BTU]], [[3btv|3BTV]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bts FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bts OCA], [http://www.ebi.ac.uk/pdbsum/3bts PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=3bts RCSB]</span>
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}}
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'''Crystal structure of a ternary complex of the transcriptional repressor Gal80p (Gal80S0 [G301R]) and the acidic activation domain of Gal4p (aa 854-874) from Saccharomyces cerevisiae with NAD'''
'''Crystal structure of a ternary complex of the transcriptional repressor Gal80p (Gal80S0 [G301R]) and the acidic activation domain of Gal4p (aa 854-874) from Saccharomyces cerevisiae with NAD'''
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[[Category: Joshua-Tor, L.]]
[[Category: Joshua-Tor, L.]]
[[Category: Kumar, P R.]]
[[Category: Kumar, P R.]]
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[[Category: acetylation]]
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[[Category: Acetylation]]
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[[Category: activator]]
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[[Category: Activator]]
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[[Category: carbohydrate metabolism]]
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[[Category: Carbohydrate metabolism]]
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[[Category: dna-binding]]
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[[Category: Dna-binding]]
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[[Category: eukaryotic transcription complex]]
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[[Category: Eukaryotic transcription complex]]
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[[Category: galactose metabolism]]
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[[Category: Galactose metabolism]]
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[[Category: metal-binding]]
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[[Category: Metal-binding]]
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[[Category: nad]]
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[[Category: Nad]]
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[[Category: nucleus]]
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[[Category: Nucleus]]
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[[Category: phosphoprotein]]
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[[Category: Phosphoprotein]]
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[[Category: repressor]]
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[[Category: Repressor]]
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[[Category: rossmann fold]]
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[[Category: Rossmann fold]]
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[[Category: transcription regulation]]
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[[Category: Transcription regulation]]
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[[Category: zinc]]
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[[Category: Zinc]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 21:05:32 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:28:53 2008''
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Revision as of 18:05, 4 May 2008

Image:3bts.jpg


PDB ID 3bts

Drag the structure with the mouse to rotate
3bts, resolution 2.70Å ()
Ligands:
Gene: GAL80 (Saccharomyces cerevisiae)
Related: 3btu, 3btv
Resources: FirstGlance, OCA, RCSB, PDBsum
Coordinates: save as pdb, mmCIF, xml



Crystal structure of a ternary complex of the transcriptional repressor Gal80p (Gal80S0 [G301R]) and the acidic activation domain of Gal4p (aa 854-874) from Saccharomyces cerevisiae with NAD


Overview

Transcriptional regulation of the galactose-metabolizing genes in Saccharomyces cerevisiae depends on three core proteins: Gal4p, the transcriptional activator that binds to upstream activating DNA sequences (UAS(GAL)); Gal80p, a repressor that binds to the carboxyl terminus of Gal4p and inhibits transcription; and Gal3p, a cytoplasmic transducer that, upon binding galactose and adenosine 5'-triphosphate, relieves Gal80p repression. The current model of induction relies on Gal3p sequestering Gal80p in the cytoplasm. However, the rapid induction of this system implies that there is a missing factor. Our structure of Gal80p in complex with a peptide from the carboxyl-terminal activation domain of Gal4p reveals the existence of a dinucleotide that mediates the interaction between the two. Biochemical and in vivo experiments suggests that nicotinamide adenine dinucleotide phosphate (NADP) plays a key role in the initial induction event.

About this Structure

3BTS is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

NADP regulates the yeast GAL induction system., Kumar PR, Yu Y, Sternglanz R, Johnston SA, Joshua-Tor L, Science. 2008 Feb 22;319(5866):1090-2. PMID:18292341 Page seeded by OCA on Sun May 4 21:05:32 2008

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