3cgt

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[[Image:3cgt.jpg|left|200px]]
[[Image:3cgt.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 3cgt |SIZE=350|CAPTION= <scene name='initialview01'>3cgt</scene>, resolution 2.4&Aring;
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The line below this paragraph, containing "STRUCTURE_3cgt", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=BCD:BETA-CYCLODEXTRIN'>BCD</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Cyclomaltodextrin_glucanotransferase Cyclomaltodextrin glucanotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.19 2.4.1.19] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_3cgt| PDB=3cgt | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3cgt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cgt OCA], [http://www.ebi.ac.uk/pdbsum/3cgt PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=3cgt RCSB]</span>
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}}
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'''STRUCTURE OF CYCLODEXTRIN GLYCOSYLTRANSFERASE COMPLEXED WITH ITS MAIN PRODUCT BETA-CYCLODEXTRIN'''
'''STRUCTURE OF CYCLODEXTRIN GLYCOSYLTRANSFERASE COMPLEXED WITH ITS MAIN PRODUCT BETA-CYCLODEXTRIN'''
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[[Category: Schmidt, A K.]]
[[Category: Schmidt, A K.]]
[[Category: Schulz, G E.]]
[[Category: Schulz, G E.]]
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[[Category: cyclodextrin]]
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[[Category: Cyclodextrin]]
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[[Category: glycosyltransferase]]
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[[Category: Glycosyltransferase]]
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[[Category: starch degradation]]
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[[Category: Starch degradation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 21:45:42 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:32:14 2008''
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Revision as of 18:45, 4 May 2008

Image:3cgt.jpg

Template:STRUCTURE 3cgt

STRUCTURE OF CYCLODEXTRIN GLYCOSYLTRANSFERASE COMPLEXED WITH ITS MAIN PRODUCT BETA-CYCLODEXTRIN


Overview

Crystals of the inactive mutant Glu257-->Ala of cyclodextrin glycosyltransferase were soaked with the cyclodextrin (CD) derivative S-(alpha-D-glucopyranosyl)-6-thio-beta-CD. The structural analysis showed its beta-CD moiety with no density indication for the exocyclic glucosyl unit. For steric reasons, however, the position of this unit is restricted to be at only two of the seven glucosyl groups of beta-CD. The analysis indicated that the enzyme can cyclize branched alpha-glucans. The ligated beta-CD moiety revealed how the enzyme binds its predominant cyclic product. The conformation of the ligated beta-CD was intermediate between the more symmetrical conformation in beta-CD dodecahydrate crystals and the conformation of a bound linear alpha-glucan chain. Its scissile bond was displaced by 2.8 A from the position in linear alpha-glucans. Accordingly, the complex represents the situation after the cyclization reaction but before diffusion into the solvent, where a more symmetrical conformation is assumed, or the equivalent state in the reverse reaction. Furthermore, a unifying nomenclature for oligosaccharide-binding subsites in proteins is proposed.

About this Structure

3CGT is a Single protein structure of sequence from Bacillus circulans. Full crystallographic information is available from OCA.

Reference

Structure of cyclodextrin glycosyltransferase complexed with a derivative of its main product beta-cyclodextrin., Schmidt AK, Cottaz S, Driguez H, Schulz GE, Biochemistry. 1998 Apr 28;37(17):5909-15. PMID:9558324 Page seeded by OCA on Sun May 4 21:45:42 2008

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