3crx

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[[Image:3crx.gif|left|200px]]
[[Image:3crx.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 3crx |SIZE=350|CAPTION= <scene name='initialview01'>3crx</scene>, resolution 2.5&Aring;
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The line below this paragraph, containing "STRUCTURE_3crx", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=CIB:Recombinase+Active+Site'>CIB</scene> and <scene name='pdbsite=CIC:Recombinase+Active+Site'>CIC</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE=
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|DOMAIN=
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{{STRUCTURE_3crx| PDB=3crx | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3crx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3crx OCA], [http://www.ebi.ac.uk/pdbsum/3crx PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=3crx RCSB]</span>
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}}
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'''CRE RECOMBINASE/DNA COMPLEX INTERMEDIATE I'''
'''CRE RECOMBINASE/DNA COMPLEX INTERMEDIATE I'''
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[[Category: Guo, F.]]
[[Category: Guo, F.]]
[[Category: Vanduyne, G D.]]
[[Category: Vanduyne, G D.]]
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[[Category: cre recombinase]]
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[[Category: Cre recombinase]]
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[[Category: holliday junction]]
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[[Category: Holliday junction]]
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[[Category: recombinase/dna complex]]
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[[Category: Recombinase/dna complex]]
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[[Category: recombination]]
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[[Category: Recombination]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 21:58:29 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:32:44 2008''
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Revision as of 18:58, 4 May 2008

Image:3crx.gif

Template:STRUCTURE 3crx

CRE RECOMBINASE/DNA COMPLEX INTERMEDIATE I


Overview

We have determined the X-ray crystal structures of two DNA Holliday junctions (HJs) bound by Cre recombinase. The HJ is a four-way branched structure that occurs as an intermediate in genetic recombination pathways, including site-specific recombination by the lambda-integrase family. Cre recombinase is an integrase family member that recombines 34 bp loxP sites in the absence of accessory proteins or auxiliary DNA sequences. The 2.7 A structure of Cre recombinase bound to an immobile HJ and the 2.5 A structure of Cre recombinase bound to a symmetric, nicked HJ reveal a nearly planar, twofold-symmetric DNA intermediate that shares features with both the stacked-X and the square conformations of the HJ that exist in the unbound state. The structures support a protein-mediated crossover isomerization of the junction that acts as the switch responsible for activation and deactivation of recombinase active sites. In this model, a subtle isomerization of the Cre recombinase-HJ quaternary structure dictates which strands are cleaved during resolution of the junction via a mechanism that involves neither branch migration nor helical restacking.

About this Structure

3CRX is a Single protein structure of sequence from Enterobacteria phage p1. Full crystallographic information is available from OCA.

Reference

Structure of the Holliday junction intermediate in Cre-loxP site-specific recombination., Gopaul DN, Guo F, Van Duyne GD, EMBO J. 1998 Jul 15;17(14):4175-87. PMID:9670032 Page seeded by OCA on Sun May 4 21:58:29 2008

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