3est

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[[Image:3est.gif|left|200px]]
[[Image:3est.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 3est |SIZE=350|CAPTION= <scene name='initialview01'>3est</scene>, resolution 1.65&Aring;
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The line below this paragraph, containing "STRUCTURE_3est", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Pancreatic_elastase Pancreatic elastase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.36 3.4.21.36] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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|DOMAIN=
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{{STRUCTURE_3est| PDB=3est | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3est FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3est OCA], [http://www.ebi.ac.uk/pdbsum/3est PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=3est RCSB]</span>
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}}
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'''STRUCTURE OF NATIVE PORCINE PANCREATIC ELASTASE AT 1.65 ANGSTROMS RESOLUTION'''
'''STRUCTURE OF NATIVE PORCINE PANCREATIC ELASTASE AT 1.65 ANGSTROMS RESOLUTION'''
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[[Category: Meyer, E F.]]
[[Category: Meyer, E F.]]
[[Category: Radhakrishnan, R.]]
[[Category: Radhakrishnan, R.]]
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[[Category: hydrolase(serine proteinase)]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 22:01:16 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:33:12 2008''
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Revision as of 19:01, 4 May 2008

Image:3est.gif

Template:STRUCTURE 3est

STRUCTURE OF NATIVE PORCINE PANCREATIC ELASTASE AT 1.65 ANGSTROMS RESOLUTION


Overview

The structure of native porcine pancreatic elastase in 70% methanol has been refined using film data to 1.65 A resolution, R = 0.169. A total of 134 molecules of water (but no methanol) has been refined. This structure, because of its native state and modestly high resolution, serves as the basis for comparison with other elastase structures complexed with natural or synthetic ligands. Internal structured water occupies distinct regions. Two regions (IW1 and IW7) suggest a mechanism for equalizing 'hydrostatic pressure' related to ligand binding and release. A third region (IW4) forms part of a hydrogen-bonding network linking the catalytic Ser 195 O gamma with a remote (13.4 A) surface of the enzyme. A comparison with the structures of all known serine proteases reveals that a linkage of Ser O gamma to remote surface is conserved in all cases, suggesting that the accepted catalytic mechanism of serine proteases needs to be re-evaluated. One possible mechanism for base catalysis of Ser O gamma H proton extraction is presented.

About this Structure

3EST is a Single protein structure of sequence from Sus scrofa. The following page contains interesting information on the relation of 3EST with [Trypsin]. Full crystallographic information is available from OCA.

Reference

Structure of native porcine pancreatic elastase at 1.65 A resolutions., Meyer E, Cole G, Radhakrishnan R, Epp O, Acta Crystallogr B. 1988 Feb 1;44 ( Pt 1):26-38. PMID:3271103 Page seeded by OCA on Sun May 4 22:01:16 2008

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