3hck
From Proteopedia
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'''NMR ENSEMBLE OF THE UNCOMPLEXED HUMAN HCK SH2 DOMAIN, 20 STRUCTURES''' | '''NMR ENSEMBLE OF THE UNCOMPLEXED HUMAN HCK SH2 DOMAIN, 20 STRUCTURES''' | ||
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[[Category: Smithgall, T E.]] | [[Category: Smithgall, T E.]] | ||
[[Category: Zhang, W.]] | [[Category: Zhang, W.]] | ||
| - | [[Category: | + | [[Category: Hck]] |
| - | [[Category: | + | [[Category: Sh2]] |
| - | [[Category: | + | [[Category: Signal transduction]] |
| - | [[Category: | + | [[Category: Transferase]] |
| - | [[Category: | + | [[Category: Tyrosine kinase]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 22:03:37 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 19:03, 4 May 2008
NMR ENSEMBLE OF THE UNCOMPLEXED HUMAN HCK SH2 DOMAIN, 20 STRUCTURES
Overview
The hematopoietic cellular kinase (Hck) is a member of the Src family of non-receptor protein-tyrosine kinases and participates in signal transduction events regulating the growth, differentiation and function of phagocytes. The secondary structure of the SH2 domain for Hck was determined for a 13C/15N-enriched sample using multi-dimensional NMR spectroscopy. The secondary structure for the domain was determined from chemical shift indices [1H alpha, 13C alpha and 13C'], sequential NOEs [d(alphaN)(i, i+1) and d(NN)(i, i+1)], and 3J(alphaN) scalar coupling constants. The Hck SH2 domain consists of two alpha-helices and seven beta-strands. Complementary strands of beta-sheets were identified from long-range NOEs using a novel 3D, 13C/15N-edited HMQC-NOESY-(HCACO)NH experiment that correlated 1H alpha resonances between beta-strands. The secondary structure for Hck SH2 is similar to that predicted from the sequence alignment of the Src-family protein tyrosine kinases.
About this Structure
3HCK is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Sequential assignment and secondary structure determination for the Src homology 2 domain of hematopoietic cellular kinase., Zhang W, Smithgall TE, Gmeiner WH, FEBS Lett. 1997 Apr 7;406(1-2):131-5. PMID:9109402 Page seeded by OCA on Sun May 4 22:03:37 2008
