3mct

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[[Image:3mct.gif|left|200px]]
[[Image:3mct.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 3mct |SIZE=350|CAPTION= <scene name='initialview01'>3mct</scene>, resolution 2.0&Aring;
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The line below this paragraph, containing "STRUCTURE_3mct", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=3MC:3-METHYLCYTOSINE'>3MC</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Polynucleotide_adenylyltransferase Polynucleotide adenylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.19 2.7.7.19] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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|DOMAIN=
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{{STRUCTURE_3mct| PDB=3mct | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3mct FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mct OCA], [http://www.ebi.ac.uk/pdbsum/3mct PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=3mct RCSB]</span>
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}}
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'''VACCINIA METHYLTRANSFERASE VP39 COMPLEXED WITH M3CYT AND S-ADENOSYLHOMOCYSTEINE'''
'''VACCINIA METHYLTRANSFERASE VP39 COMPLEXED WITH M3CYT AND S-ADENOSYLHOMOCYSTEINE'''
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[[Category: Hu, G.]]
[[Category: Hu, G.]]
[[Category: Quiocho, F A.]]
[[Category: Quiocho, F A.]]
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[[Category: complex (transferase/rna cap analog)]]
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[[Category: Methylated cytosine]]
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[[Category: methylated cytosine]]
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[[Category: Methyltransferase]]
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[[Category: methyltransferase]]
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[[Category: Mrna processing]]
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[[Category: mrna processing]]
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[[Category: Rna cap analog]]
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[[Category: poly(a) polymerase]]
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[[Category: Transcription]]
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[[Category: rna cap analog]]
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[[Category: Vaccinia]]
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[[Category: transcription]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 22:07:01 2008''
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[[Category: vaccinia]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:34:35 2008''
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Revision as of 19:07, 4 May 2008

Image:3mct.gif

Template:STRUCTURE 3mct

VACCINIA METHYLTRANSFERASE VP39 COMPLEXED WITH M3CYT AND S-ADENOSYLHOMOCYSTEINE


Overview

We have determined, by high resolution x-ray analysis, 10 structures comprising the mRNA cap-specific methyltransferase VP39 or specific mutants thereof in the presence of methylated nucleobase analogs (N1-methyladenine, N3-methyladenine, N1-methylcytosine, N3-methylcytosine) and their unmethylated counterparts, or nucleoside N7-methylguanosine. Together with solution affinity studies and previous crystallographic data for N7-methylguanosine and its phosphorylated derivatives, these data demonstrate that only methylated, positively charged bases are bound, indicating that their enhanced stacking with two aromatic side chains of VP39 (Tyr 22 and Phe 180) plays a dominant role in cap recognition. Four key features characterize this stacking interaction: (i) near perfect parallel alignment between the sandwiched methylated bases and aromatic side chains, (ii) substantial areas of overlap in the two-stacked rings, (iii) a 3.4-A interplanar spacing within the overlapping region, and (iv) positive charge in the heterocyclic nucleobase.

About this Structure

3MCT is a Single protein structure of sequence from Vaccinia virus. Full crystallographic information is available from OCA.

Reference

mRNA cap recognition: dominant role of enhanced stacking interactions between methylated bases and protein aromatic side chains., Hu G, Gershon PD, Hodel AE, Quiocho FA, Proc Natl Acad Sci U S A. 1999 Jun 22;96(13):7149-54. PMID:10377383 Page seeded by OCA on Sun May 4 22:07:01 2008

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