3p2p

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[[Image:3p2p.jpg|left|200px]]
[[Image:3p2p.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 3p2p |SIZE=350|CAPTION= <scene name='initialview01'>3p2p</scene>, resolution 2.1&Aring;
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The line below this paragraph, containing "STRUCTURE_3p2p", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Phospholipase_A(2) Phospholipase A(2)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.4 3.1.1.4] </span>
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{{STRUCTURE_3p2p| PDB=3p2p | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3p2p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3p2p OCA], [http://www.ebi.ac.uk/pdbsum/3p2p PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=3p2p RCSB]</span>
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'''ENHANCED ACTIVITY AND ALTERED SPECIFICITY OF PHOSPHOLIPASE A2 BY DELETION OF A SURFACE LOOP'''
'''ENHANCED ACTIVITY AND ALTERED SPECIFICITY OF PHOSPHOLIPASE A2 BY DELETION OF A SURFACE LOOP'''
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==Reference==
==Reference==
Enhanced activity and altered specificity of phospholipase A2 by deletion of a surface loop., Kuipers OP, Thunnissen MM, de Geus P, Dijkstra BW, Drenth J, Verheij HM, de Haas GH, Science. 1989 Apr 7;244(4900):82-5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/2704992 2704992]
Enhanced activity and altered specificity of phospholipase A2 by deletion of a surface loop., Kuipers OP, Thunnissen MM, de Geus P, Dijkstra BW, Drenth J, Verheij HM, de Haas GH, Science. 1989 Apr 7;244(4900):82-5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/2704992 2704992]
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[[Category: Phospholipase A(2)]]
 
[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Sus scrofa]]
[[Category: Sus scrofa]]
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[[Category: Kalk, K H.]]
[[Category: Kalk, K H.]]
[[Category: Thunnissen, M M.G M.]]
[[Category: Thunnissen, M M.G M.]]
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[[Category: hydrolase(carboxyl ester)]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 22:08:41 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:34:59 2008''
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Revision as of 19:08, 4 May 2008

Image:3p2p.jpg

Template:STRUCTURE 3p2p

ENHANCED ACTIVITY AND ALTERED SPECIFICITY OF PHOSPHOLIPASE A2 BY DELETION OF A SURFACE LOOP


Overview

Protein engineering and x-ray crystallography have been used to study the role of a surface loop that is present in pancreatic phospholipases but is absent in snake venom phospholipases. Removal of residues 62 to 66 from porcine pancreatic phospholipase A2 does not change the binding constant for micelles significantly, but it improves catalytic activity up to 16 times on micellar (zwitterionic) lecithin substrates. In contrast, the decrease in activity on negatively charged substrates is greater than fourfold. A crystallographic study of the mutant enzyme shows that the region of the deletion has a well-defined structure that differs from the structure of the wild-type enzyme. No structural changes in the active site of the enzyme were detected.

About this Structure

3P2P is a Single protein structure of sequence from Sus scrofa. Full crystallographic information is available from OCA.

Reference

Enhanced activity and altered specificity of phospholipase A2 by deletion of a surface loop., Kuipers OP, Thunnissen MM, de Geus P, Dijkstra BW, Drenth J, Verheij HM, de Haas GH, Science. 1989 Apr 7;244(4900):82-5. PMID:2704992 Page seeded by OCA on Sun May 4 22:08:41 2008

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