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439d
From Proteopedia
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[[Image:439d.gif|left|200px]] | [[Image:439d.gif|left|200px]] | ||
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| - | + | {{STRUCTURE_439d| PDB=439d | SCENE= }} | |
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'''5'-R(*CP*UP*GP*GP*GP*CP*GP*G)-3', 5'-R(*CP*CP*GP*CP*CP*UP*GP*G)-3'''' | '''5'-R(*CP*UP*GP*GP*GP*CP*GP*G)-3', 5'-R(*CP*CP*GP*CP*CP*UP*GP*G)-3'''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=439D OCA]. | |
==Reference== | ==Reference== | ||
Structure of an RNA duplex with an unusual G.C pair in wobble-like conformation at 1.6 A resolution., Perbandt M, Vallazza M, Lippmann C, Betzel C, Erdmann VA, Acta Crystallogr D Biol Crystallogr. 2001 Feb;57(Pt 2):219-24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11173467 11173467] | Structure of an RNA duplex with an unusual G.C pair in wobble-like conformation at 1.6 A resolution., Perbandt M, Vallazza M, Lippmann C, Betzel C, Erdmann VA, Acta Crystallogr D Biol Crystallogr. 2001 Feb;57(Pt 2):219-24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11173467 11173467] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Betzel, C.]] | [[Category: Betzel, C.]] | ||
[[Category: Erdmann, V A.]] | [[Category: Erdmann, V A.]] | ||
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[[Category: Perbandt, M.]] | [[Category: Perbandt, M.]] | ||
[[Category: Vallazza, M.]] | [[Category: Vallazza, M.]] | ||
| - | [[Category: | + | [[Category: A-rna]] |
| - | [[Category: | + | [[Category: Fragment of 5s rrna]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 22:17:53 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 19:17, 4 May 2008
5'-R(*CP*UP*GP*GP*GP*CP*GP*G)-3', 5'-R(*CP*CP*GP*CP*CP*UP*GP*G)-3'
Overview
The structure of the RNA duplex r(CUGGGCGG).r(CCGCCUGG) has been determined at 1.6 A resolution and refined to a final R factor of 18.3% (R(free) = 24.1%). The sequence of the RNA fragment resembles domain E of Thermus flavus 5S rRNA. A previously undescribed wobble-like G.C base-pair formation is found. Owing to the observed hydrogen-bond network, it is proposed that the cytosine is protonated at position N3. The unusual base-pair formation is presumably strained by intermolecular interactions. In this context, crystal packing and particular intermolecular contacts may have direct influence on the three-dimensional structure. Furthermore, this structure includes two G.U wobble base pairs in tandem conformation, with the purines forming a so-called 'cross-strand G stack'.
About this Structure
Full crystallographic information is available from OCA.
Reference
Structure of an RNA duplex with an unusual G.C pair in wobble-like conformation at 1.6 A resolution., Perbandt M, Vallazza M, Lippmann C, Betzel C, Erdmann VA, Acta Crystallogr D Biol Crystallogr. 2001 Feb;57(Pt 2):219-24. PMID:11173467 Page seeded by OCA on Sun May 4 22:17:53 2008
