1axh

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ATRACOTOXIN-HVI FROM HADRONYCHE VERSUTA (AUSTRALIAN FUNNEL-WEB SPIDER, NMR, 20 STRUCTURES

Overview

A family of potent insecticidal toxins has recently been isolated from the, venom of Australian funnel web spiders. Among these is the 37-residue, peptide omega-atracotoxin-HV1 (omega-ACTX-HV1) from Hadronyche versuta. We, have chemically synthesized and folded omega-ACTX-HV1, shown that it is, neurotoxic, ascertained its disulphide bonding pattern, and determined its, three-dimensional solution structure using NMR spectroscopy. The structure, consists of a solvent-accessible beta-hairpin protruding from a, disulphide-bonded globular core comprising four beta-turns. The three, intramolecular disulphide bonds from a cystine knot motif similar to that, seen in several other neurotoxic peptides. Despite limited sequence, identity, omega-ACTX-HV1 displays significant structural homology with the, omega-agatoxins and omega-conotoxins, both of which are vertebrate calcium, channel antagonists; however, in contrast with these toxins, we show that, omega-ACTX-HV1 inhibits insect, but not mammalian, voltage-gated calcium, channel currents.

About this Structure

1AXH is a Single protein structure of sequence from Hadronyche versuta. Full crystallographic information is available from OCA.

Reference

The structure of a novel insecticidal neurotoxin, omega-atracotoxin-HV1, from the venom of an Australian funnel web spider., Fletcher JI, Smith R, O'Donoghue SI, Nilges M, Connor M, Howden ME, Christie MJ, King GF, Nat Struct Biol. 1997 Jul;4(7):559-66. PMID:9228949

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