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1axk
From Proteopedia
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(New page: 200px<br /><applet load="1axk" size="450" color="white" frame="true" align="right" spinBox="true" caption="1axk, resolution 2.1Å" /> '''ENGINEERED BACILLUS B...)
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Revision as of 09:04, 20 November 2007
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ENGINEERED BACILLUS BIFUNCTIONAL ENZYME GLUXYN-1
Overview
The 1,3-1,4-beta-glucanase from Bacillus macerans (wtGLU) and the 1, 4-beta-xylanase from Bacillus subtilis (wtXYN) are both single-domain, jellyroll proteins catalyzing similar enzymatic reactions. In the fusion, protein GluXyn-1, the two proteins are joined by insertion of the entire, XYN domain into a surface loop of cpMAC-57, a circularly permuted variant, of wtGLU. GluXyn-1 was generated by protein engineering methods, produced, in Escherichia coli and shown to fold spontaneously and have both, enzymatic activities at wild-type level. The crystal structure of GluXyn-1, was determined at 2.1 A resolution and refined to R = 17.7% and R(free) =, 22.4%. It shows nearly ideal, native-like folding of both protein domains, and a small, but significant hinge bending between the domains. The active, sites are independent and accessible explaining the observed enzymatic, activity. Because in GluXyn-1 the complete XYN domain is inserted into the, compact folding unit of GLU, the wild-type-like activity and tertiary, structure of the latter proves that the folding process of GLU does not, depend on intramolecular interactions that are short-ranged in the, sequence. Insertion fusions of the GluXyn-1 type may prove to be an easy, route toward more stable bifunctional proteins in which the two parts are, more closely associated than in linear end-to-end protein fusions.
About this Structure
1AXK is a Single protein structure of sequence from Bacillus subtilis with CA as ligand. Full crystallographic information is available from OCA.
Reference
Structure and function of the Bacillus hybrid enzyme GluXyn-1: native-like jellyroll fold preserved after insertion of autonomous globular domain., Ay J, Gotz F, Borriss R, Heinemann U, Proc Natl Acad Sci U S A. 1998 Jun 9;95(12):6613-8. PMID:9618460
Page seeded by OCA on Tue Nov 20 11:11:18 2007
Categories: Bacillus subtilis | Single protein | Ay, J. | Heinemann, U. | CA | 1 | 3-1 | 4-beta-glucanase | 4-beta-xylanase | Bifunctional | Fusion protein | Gluxyn-1 | Hybrid enzyme
