469d
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:469d.gif|left|200px]] | [[Image:469d.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_469d", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | | | + | or leave the SCENE parameter empty for the default display. |
| - | | | + | --> |
| - | + | {{STRUCTURE_469d| PDB=469d | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''CRYSTAL STRUCTURE AND IMPROVED ANTISENSE PROPERTIES OF 2'-O-(2-METHOXYETHYL)-RNA''' | '''CRYSTAL STRUCTURE AND IMPROVED ANTISENSE PROPERTIES OF 2'-O-(2-METHOXYETHYL)-RNA''' | ||
| Line 19: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=469D OCA]. | |
==Reference== | ==Reference== | ||
Crystal structure and improved antisense properties of 2'-O-(2-methoxyethyl)-RNA., Teplova M, Minasov G, Tereshko V, Inamati GB, Cook PD, Manoharan M, Egli M, Nat Struct Biol. 1999 Jun;6(6):535-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10360355 10360355] | Crystal structure and improved antisense properties of 2'-O-(2-methoxyethyl)-RNA., Teplova M, Minasov G, Tereshko V, Inamati GB, Cook PD, Manoharan M, Egli M, Nat Struct Biol. 1999 Jun;6(6):535-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10360355 10360355] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Cook, P D.]] | [[Category: Cook, P D.]] | ||
[[Category: Egli, M.]] | [[Category: Egli, M.]] | ||
| Line 30: | Line 26: | ||
[[Category: Teplova, M.]] | [[Category: Teplova, M.]] | ||
[[Category: Tereshko, V.]] | [[Category: Tereshko, V.]] | ||
| - | [[Category: | + | [[Category: Rna dodecamer duplex]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 22:19:22 2008'' | |
| - | + | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 19:19, 4 May 2008
CRYSTAL STRUCTURE AND IMPROVED ANTISENSE PROPERTIES OF 2'-O-(2-METHOXYETHYL)-RNA
Overview
2'-O-(2-Methoxyethyl)-RNA (MOE-RNA) is a nucleic acid analog with promising features for antisense applications. Compared with phosphorothioate DNA (PS-DNA), the MOE modification offers improved nuclease resistance, enhanced RNA affinity, improved cellular uptake and intestinal absorption, reduced toxicity and immune stimulation. The crystal structure of a fully modified MOE-RNA dodecamer duplex (CGCGAAUUCGCG) was determined at 1.7 A resolution. In the majority of the MOE substituents, the torsion angle around the ethylene alkyl chain assumes a gauche conformation. The conformational preorganization of the MOE groups is consistent with the improved RNA affinity and the extensive hydration of the substituents could play a role in the improved cellular uptake of MOE-RNA. A specific hydration pattern that bridges substituent and phosphate oxygen atoms in the minor groove of MOE-RNA may explain its high nuclease resistance.
About this Structure
Full crystallographic information is available from OCA.
Reference
Crystal structure and improved antisense properties of 2'-O-(2-methoxyethyl)-RNA., Teplova M, Minasov G, Tereshko V, Inamati GB, Cook PD, Manoharan M, Egli M, Nat Struct Biol. 1999 Jun;6(6):535-9. PMID:10360355 Page seeded by OCA on Sun May 4 22:19:22 2008
