473d
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:473d.gif|left|200px]] | [[Image:473d.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_473d", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | + | --> | |
| - | | | + | {{STRUCTURE_473d| PDB=473d | SCENE= }} |
| - | | | + | |
| - | + | ||
| - | }} | + | |
'''NI2+/GUANINE INTERACTIONS AND NETROPSIN/GUANINE STACKING IN D(CGTATATACG)2''' | '''NI2+/GUANINE INTERACTIONS AND NETROPSIN/GUANINE STACKING IN D(CGTATATACG)2''' | ||
| Line 19: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=473D OCA]. | |
==Reference== | ==Reference== | ||
Stacking interaction of guanine with netropsin in the minor groove of d(CGTATATACG)2., Abrescia NG, Malinina L, Subirana JA, J Mol Biol. 1999 Dec 3;294(3):657-66. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10610787 10610787] | Stacking interaction of guanine with netropsin in the minor groove of d(CGTATATACG)2., Abrescia NG, Malinina L, Subirana JA, J Mol Biol. 1999 Dec 3;294(3):657-66. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10610787 10610787] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Abrescia, N G.A.]] | [[Category: Abrescia, N G.A.]] | ||
[[Category: Malinina, L.]] | [[Category: Malinina, L.]] | ||
[[Category: Subirana, J A.]] | [[Category: Subirana, J A.]] | ||
| - | [[Category: | + | [[Category: Minor groove binding]] |
| - | [[Category: | + | [[Category: Netropsin/guanine stacking]] |
| - | [[Category: | + | [[Category: Ni2+/guanine interaction]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 22:19:33 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 19:19, 4 May 2008
NI2+/GUANINE INTERACTIONS AND NETROPSIN/GUANINE STACKING IN D(CGTATATACG)2
Overview
We present the structure of the decanucleotide d(CGTATATACG) determined by single crystal X-ray diffraction at 1.58 A resolution. A netropsin drug is found in the minor groove with guanine stacked on a pyrrole ring of the drug, a feature described here for the first time. The stacked guanine is an extra-helical base coming from the end of a neighbour oligonucleotide. This observation may open the way to the development of minor groove binding drugs with a higher sequence selectivity. The oligonucleotide is in the B-conformation, but the terminal base-pairs are disrupted: the cytosine residues are disordered while the guanine residues penetrate into the minor groove of neighbouring duplexes. Four hydrated Ni ions with octahedral co-ordination are found associated with the N7 atoms of each guanine. The high affinity of these ions with guanine suggests that they may be used as probes for specific guanine residues.
About this Structure
Full crystallographic information is available from OCA.
Reference
Stacking interaction of guanine with netropsin in the minor groove of d(CGTATATACG)2., Abrescia NG, Malinina L, Subirana JA, J Mol Biol. 1999 Dec 3;294(3):657-66. PMID:10610787 Page seeded by OCA on Sun May 4 22:19:33 2008
