4aah
From Proteopedia
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[[Image:4aah.gif|left|200px]] | [[Image:4aah.gif|left|200px]] | ||
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'''METHANOL DEHYDROGENASE FROM METHYLOPHILUS W3A1''' | '''METHANOL DEHYDROGENASE FROM METHYLOPHILUS W3A1''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | 4AAH is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Methylophilus Methylophilus]. This structure supersedes the now removed PDB entry | + | 4AAH is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Methylophilus Methylophilus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3aah 3aah]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AAH OCA]. |
==Reference== | ==Reference== | ||
Determination of the gene sequence and the three-dimensional structure at 2.4 angstroms resolution of methanol dehydrogenase from Methylophilus W3A1., Xia Z, Dai W, Zhang Y, White SA, Boyd GD, Mathews FS, J Mol Biol. 1996 Jun 14;259(3):480-501. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8676383 8676383] | Determination of the gene sequence and the three-dimensional structure at 2.4 angstroms resolution of methanol dehydrogenase from Methylophilus W3A1., Xia Z, Dai W, Zhang Y, White SA, Boyd GD, Mathews FS, J Mol Biol. 1996 Jun 14;259(3):480-501. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8676383 8676383] | ||
| - | [[Category: Alcohol dehydrogenase (acceptor)]] | ||
[[Category: Methylophilus]] | [[Category: Methylophilus]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Mathews, F S.]] | [[Category: Mathews, F S.]] | ||
[[Category: Xia, Z X.]] | [[Category: Xia, Z X.]] | ||
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 22:20:28 2008'' | |
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Revision as of 19:20, 4 May 2008
METHANOL DEHYDROGENASE FROM METHYLOPHILUS W3A1
Overview
The DNA sequences for the genes encoding the heavy and light subunits of methanol dehydrogenase from Methylophilus methylotrophus W3A1 have been determined. The deduced amino acid sequence has enabled the structure of the enzyme to be refined at 2.4 angstrom resolution against X-ray data collected on a Hamlin area detector. The structure was refined using the programs PROFFT and X-PLOR with several model building step interspersed. The final model contains two heavy chains (571 amino acids), two light chains (69 amino acids), two molecules of pyrroloquinoline quinone, two Ca2+ and 521 solvent molecules. Each half molecule contains four disulfide linkages and four cis peptides. One of the disulfides is formed from two adjacent cysteine residues linked by a trans peptide which creates a novel eight-membered ring. The heavy subunit is an 8-fold beta-propeller, each "blade" of which is a four-stranded antiparallel twisted beta-sheet. The light chain is an elongated subunit stretching across the surface of the heavy subunit, with residues 1 to 32 containing four beta-turns and residues 33 to 62 forming a helix; however, it neither interacts with the active site, nor the other HL dimer and its functional role is obscure. Around the 8-fold beta-propeller there is a repeating pattern of tryptophan residues located in the outer strand of seven of the eight beta-leaflets, each packed between adjacent leaflets. Each of these tryptophan residues is centered in the beta-strand and participates in the main chain hydrogen bonding of the sheet. Five of the seven tryptophan residues have closely similar interactions with the adjacent beta-leaflet including stacking of the tryptophan indole rings against a peptide plane and formation of a hydrogen bond from NE1 of the indole ring to a main-chain carbonyl. This repeating pattern is conserved over a number of MEDH sequences. The PQQ is located on the pseudo 8-fold rotation axis of the heavy subunit, in a funnel-shaped internal cavity, sandwiched between the indole ring of Trp237 and the two sulfur atoms of the Cys103-Cys104 vicinal disulfide. A hexacoordinate Ca2+ is bound in the active site by one nitrogen and five oxygen ligands, three from the PQQ and the others from two protein side-chains. In the active site an isolated solvent molecule is bound to the O5 of PQQ and to a nearby aspartate side-chain; its position may be the binding site for methanol. The aspartate might than serve as a general base for proton abstraction from the substrate hydroxyl. The C5 atom of PQQ could be activated by electrophilic catalysis by a nearby argenine side-chain or by the calcium ion bound to PQQ.
About this Structure
4AAH is a Protein complex structure of sequences from Methylophilus. This structure supersedes the now removed PDB entry 3aah. Full crystallographic information is available from OCA.
Reference
Determination of the gene sequence and the three-dimensional structure at 2.4 angstroms resolution of methanol dehydrogenase from Methylophilus W3A1., Xia Z, Dai W, Zhang Y, White SA, Boyd GD, Mathews FS, J Mol Biol. 1996 Jun 14;259(3):480-501. PMID:8676383 Page seeded by OCA on Sun May 4 22:20:28 2008
