4eng
From Proteopedia
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'''STRUCTURE OF ENDOGLUCANASE V CELLOHEXAOSE COMPLEX''' | '''STRUCTURE OF ENDOGLUCANASE V CELLOHEXAOSE COMPLEX''' | ||
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[[Category: Davies, G J.]] | [[Category: Davies, G J.]] | ||
[[Category: Schulein, M.]] | [[Category: Schulein, M.]] | ||
| - | [[Category: | + | [[Category: Endoglucanase]] |
| - | [[Category: | + | [[Category: Glycosyl hydrolase]] |
| - | [[Category: | + | [[Category: Hydrolase]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 22:23:16 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 19:23, 4 May 2008
STRUCTURE OF ENDOGLUCANASE V CELLOHEXAOSE COMPLEX
Overview
The structure of the catalytic core of the endoglucanase V (EGV) from Humicola insolens has been determined by the method of multiple isomorphous replacement at 1.5 A resolution. The final model, refined with X-PLOR and PROLSQ, has a crystallographic R factor of 0.163 (R(free) = 0.240) with deviations from stereochemical target values of 0.012 A and 0.037 degrees for bonds and angles, respectively. The model was further refined with SHELXL, including anisotropic modelling of the protein-atom temperature factors, to give a final model with an R factor of 0.105 and an R(free) of 0.154. The initial isomorphous replacement electron-density map was poor and uninterpretable but was improved by the use of synchrotron data collected at a wavelength chosen so as to optimize the f" contribution of the anomalous scattering from the heavy atoms. The structure of H. insolens EGV consists of a six-stranded beta-barrel domain, similar to that found in a family of plant defence proteins, linked by a number of disulfide-bonded loop regions. A long open groove runs across the surface of the enzyme either side of which lie the catalytic aspartate residues. The 9 A separation of the catalytic carboxylate groups is consistent with the observation that EGV catalyzes the hydrolysis of the cellulose, beta(1-->4) links with inversion of configuration at the anomeric C1 atom. This structure is the first representative from the glycosyl hydrolase family 45.
About this Structure
4ENG is a Single protein structure of sequence from Humicola insolens. Full crystallographic information is available from OCA.
Reference
Structure determination and refinement of the Humicola insolens endoglucanase V at 1.5 A resolution., Davies GJ, Dodson G, Moore MH, Tolley SP, Dauter Z, Wilson KS, Rasmussen G, Schulein M, Acta Crystallogr D Biol Crystallogr. 1996 Jan 1;52(Pt 1):7-17. PMID:15299721 Page seeded by OCA on Sun May 4 22:23:16 2008
