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4eca

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[[Image:4eca.jpg|left|200px]]
[[Image:4eca.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 4eca |SIZE=350|CAPTION= <scene name='initialview01'>4eca</scene>, resolution 2.2&Aring;
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The line below this paragraph, containing "STRUCTURE_4eca", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=AS:Active+Site+In+Monomer+A'>AS</scene>, <scene name='pdbsite=BS:Active+Site+In+Monomer+B'>BS</scene>, <scene name='pdbsite=CS:Active+Site+In+Monomer+C'>CS</scene> and <scene name='pdbsite=DS:Active+Site+In+Monomer+D'>DS</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=AEI:THREONINE-ASPARTIC+ESTER'>AEI</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Asparaginase Asparaginase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.1 3.5.1.1] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= ANSB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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-->
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|DOMAIN=
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{{STRUCTURE_4eca| PDB=4eca | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4eca FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eca OCA], [http://www.ebi.ac.uk/pdbsum/4eca PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=4eca RCSB]</span>
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}}
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'''ASPARAGINASE FROM E. COLI, MUTANT T89V WITH COVALENTLY BOUND ASPARTATE'''
'''ASPARAGINASE FROM E. COLI, MUTANT T89V WITH COVALENTLY BOUND ASPARTATE'''
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[[Category: Palm, G J.]]
[[Category: Palm, G J.]]
[[Category: Wlodawer, A.]]
[[Category: Wlodawer, A.]]
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[[Category: acyl-enzyme intermediate]]
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[[Category: Acyl-enzyme intermediate]]
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[[Category: hydrolase]]
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[[Category: Hydrolase]]
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[[Category: threonine amidohydrolase]]
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[[Category: Threonine amidohydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 22:23:17 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:38:33 2008''
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Revision as of 19:23, 4 May 2008

Image:4eca.jpg

Template:STRUCTURE 4eca

ASPARAGINASE FROM E. COLI, MUTANT T89V WITH COVALENTLY BOUND ASPARTATE


Overview

Escherichia coli asparaginase II catalyzes the hydrolysis of L-asparagine to L-aspartate via a threonine-bound acyl-enzyme intermediate. A nearly inactive mutant in which one of the active site threonines, Thr-89, was replaced by valine was constructed, expressed, and crystallized. Its structure, solved at 2.2 A resolution, shows high overall similarity to the wild-type enzyme, but an aspartyl moiety is covalently bound to Thr-12, resembling a reaction intermediate. Kinetic analysis confirms the deacylation deficiency, which is also explained on a structural basis. The previously identified oxyanion hole is described in more detail.

About this Structure

4ECA is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

A covalently bound catalytic intermediate in Escherichia coli asparaginase: crystal structure of a Thr-89-Val mutant., Palm GJ, Lubkowski J, Derst C, Schleper S, Rohm KH, Wlodawer A, FEBS Lett. 1996 Jul 22;390(2):211-6. PMID:8706862 Page seeded by OCA on Sun May 4 22:23:17 2008

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