4gat

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[[Image:4gat.jpg|left|200px]]
[[Image:4gat.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 4gat |SIZE=350|CAPTION= <scene name='initialview01'>4gat</scene>
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The line below this paragraph, containing "STRUCTURE_4gat", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE= POTENTIAL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=162425 Emericella nidulans])
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|DOMAIN=
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{{STRUCTURE_4gat| PDB=4gat | SCENE= }}
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|RELATEDENTRY=[[5gat|5GAT]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gat FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gat OCA], [http://www.ebi.ac.uk/pdbsum/4gat PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=4gat RCSB]</span>
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}}
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'''SOLUTION NMR STRUCTURE OF THE WILD TYPE DNA BINDING DOMAIN OF AREA COMPLEXED TO A 13BP DNA CONTAINING A CGATA SITE, REGULARIZED MEAN STRUCTURE'''
'''SOLUTION NMR STRUCTURE OF THE WILD TYPE DNA BINDING DOMAIN OF AREA COMPLEXED TO A 13BP DNA CONTAINING A CGATA SITE, REGULARIZED MEAN STRUCTURE'''
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[[Category: Starich, M.]]
[[Category: Starich, M.]]
[[Category: Wikstrom, M.]]
[[Category: Wikstrom, M.]]
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[[Category: complex (transcription regulation/dna)]]
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[[Category: Dna binding protein]]
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[[Category: dna binding protein]]
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[[Category: Transcription factor]]
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[[Category: transcription factor]]
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[[Category: Zinc binding domain]]
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[[Category: zinc binding domain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 22:24:21 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:38:52 2008''
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Revision as of 19:24, 4 May 2008

Template:STRUCTURE 4gat

SOLUTION NMR STRUCTURE OF THE WILD TYPE DNA BINDING DOMAIN OF AREA COMPLEXED TO A 13BP DNA CONTAINING A CGATA SITE, REGULARIZED MEAN STRUCTURE


Overview

The solution structure of a complex between the DNA binding domain of a fungal GATA factor and a 13 base-pair oligonucleotide containing its physiologically relevant CGATAG target sequence has been determined by multidimensional nuclear magnetic resonance spectroscopy. The AREA DNA binding domain, from Aspergillus nidulans, possesses a single Cys2-Cys2 zinc finger module and a basic C-terminal tail, which recognize the CGATAG element via an extensive network of hydrophobic interactions with the bases in the major groove and numerous non-specific contacts along the sugar-phosphate backbone. The zinc finger core of the AREA DNA binding domain has the same global fold as that of the C-terminal DNA binding domain of chicken GATA-1. In contrast to the complex with the DNA binding domain of GATA-1 in which the basic C-terminal tail wraps around the DNA and lies in the minor groove, the structure of complex with the AREA DNA binding domain reveals that the C-terminal tail of the fungal domain runs parallel with the sugar phosphate backbone along the edge of the minor groove. This difference is principally attributed to amino acid substitutions at two positions of the AREA DNA binding domain (Val55, Asn62) relative to that of GATA-1 (Gly55, Lys62). The impact of the different C-terminal tail binding modes on the affinity and specificity of GATA factors is discussed.

About this Structure

4GAT is a Single protein structure of sequence from Emericella nidulans. Full crystallographic information is available from OCA.

Reference

The solution structure of a fungal AREA protein-DNA complex: an alternative binding mode for the basic carboxyl tail of GATA factors., Starich MR, Wikstrom M, Arst HN Jr, Clore GM, Gronenborn AM, J Mol Biol. 1998 Apr 3;277(3):605-20. PMID:9533883 Page seeded by OCA on Sun May 4 22:24:21 2008

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