4pbg

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:4pbg.gif|left|200px]]
[[Image:4pbg.gif|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 4pbg |SIZE=350|CAPTION= <scene name='initialview01'>4pbg</scene>, resolution 2.5&Aring;
+
The line below this paragraph, containing "STRUCTURE_4pbg", creates the "Structure Box" on the page.
-
|SITE= <scene name='pdbsite=PHA:Phosphate+Binding+Site'>PHA</scene> and <scene name='pdbsite=PHB:Phosphate+Binding+Site'>PHB</scene>
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND= <scene name='pdbligand=BGP:BETA-GALACTOSE-6-PHOSPHATE'>BGP</scene>
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/6-phospho-beta-galactosidase 6-phospho-beta-galactosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.85 3.2.1.85] </span>
+
or leave the SCENE parameter empty for the default display.
-
|GENE= LACG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1358 Lactococcus lactis])
+
-->
-
|DOMAIN=
+
{{STRUCTURE_4pbg| PDB=4pbg | SCENE= }}
-
|RELATEDENTRY=
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pbg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pbg OCA], [http://www.ebi.ac.uk/pdbsum/4pbg PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=4pbg RCSB]</span>
+
-
}}
+
'''6-PHOSPHO-BETA-GALACTOSIDASE FORM-CST'''
'''6-PHOSPHO-BETA-GALACTOSIDASE FORM-CST'''
Line 28: Line 25:
[[Category: Schulz, G E.]]
[[Category: Schulz, G E.]]
[[Category: Wiesmann, C.]]
[[Category: Wiesmann, C.]]
-
[[Category: glycosyl hydrolase]]
+
[[Category: Glycosyl hydrolase]]
-
[[Category: hydrolase]]
+
[[Category: Hydrolase]]
-
 
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 22:27:54 2008''
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:39:47 2008''
+

Revision as of 19:27, 4 May 2008

Image:4pbg.gif

Template:STRUCTURE 4pbg

6-PHOSPHO-BETA-GALACTOSIDASE FORM-CST


Overview

The initial structural model of 6-phospho-beta-galactosidase from Lactococcus lactis was refined to an R-factor of 16.4% (R[free] = 23.6%) to 2.3 A resolution (1 A = 0.1 nm), and the structures of three other crystal forms were solved by molecular replacement. The four structural models are essentially identical. The catalytic center of the enzyme is approximately at the mass center of the molecule and can only be reached through a 20 A long channel, which is observed with an "open" or "closed" entrance. The closed entrance is probably too small for the educt lactose-6-phosphate to enter, but large enough for the first product glucose to leave. Among the presented structures is a complex between an almost inactive mutant and the second product galactose-6-phosphate, which is exclusively bound at side-chains. A superposition (onto the native enzyme) of galactose-6-phosphate as bound to the mutant suggests the geometry of a postulated covalent intermediate. The binding mode of the educt was modeled, starting from the bound galactose-6-phosphate. A tightly fixed tryptophan is used as a chopping-board for splitting the disaccharide, and several other aromatic residues in the active center cavity are likely to participate in substrate transport/binding.

About this Structure

4PBG is a Single protein structure of sequence from Lactococcus lactis. Full crystallographic information is available from OCA.

Reference

Crystal structures and mechanism of 6-phospho-beta-galactosidase from Lactococcus lactis., Wiesmann C, Hengstenberg W, Schulz GE, J Mol Biol. 1997 Jun 27;269(5):851-60. PMID:9223646 Page seeded by OCA on Sun May 4 22:27:54 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4pbg"
Personal tools