4rub

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[[Image:4rub.gif|left|200px]]
[[Image:4rub.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 4rub |SIZE=350|CAPTION= <scene name='initialview01'>4rub</scene>, resolution 2.7&Aring;
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The line below this paragraph, containing "STRUCTURE_4rub", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CAP:2-CARBOXYARABINITOL-1,5-DIPHOSPHATE'>CAP</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribulose-bisphosphate_carboxylase Ribulose-bisphosphate carboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.39 4.1.1.39] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_4rub| PDB=4rub | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rub FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rub OCA], [http://www.ebi.ac.uk/pdbsum/4rub PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=4rub RCSB]</span>
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}}
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'''A CRYSTAL FORM OF RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE(SLASH)OXYGENASE FROM NICOTIANA TABACUM IN THE ACTIVATED STATE'''
'''A CRYSTAL FORM OF RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE(SLASH)OXYGENASE FROM NICOTIANA TABACUM IN THE ACTIVATED STATE'''
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[[Category: Eisenberg, D.]]
[[Category: Eisenberg, D.]]
[[Category: Schreuder, H.]]
[[Category: Schreuder, H.]]
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[[Category: lyase(carbon-carbon)]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 22:29:08 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:40:05 2008''
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Revision as of 19:29, 4 May 2008

Image:4rub.gif

Template:STRUCTURE 4rub

A CRYSTAL FORM OF RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE(SLASH)OXYGENASE FROM NICOTIANA TABACUM IN THE ACTIVATED STATE


Overview

A new crystal form of ribulose-1,5-bisphosphate carboxylase/oxygenase (EC 4.1.1.39) from Nicotiana tabacum has been obtained at alkaline pH with polyethylene glycol 8000 in the presence of a non-ionic detergent, beta-octyl glucoside. The crystals are grown at room temperature by the hanging-drop vapor diffusion technique from a protein solution containing enzyme complexed with CO2, Mg2+, and the transition state analog 2-C-carboxy-D-arabinitol-1,5-bisphosphate. The crystals belong to the the space group P3(1)21 (or P3(2)21) with the cell parameters a = 204.6 A, and c = 117.4 A (1 A = 0.1 nm). The asymmetric unit contains half (L4S4: L, large subunit, 53,000 Mr; S, small subunit, 15,000 Mr) of a hexadecameric molecule (L8S8, 540,000 Mr). The crystals diffract to at least 2.6 A Bragg spacing and are suitable for X-ray structure determination.

About this Structure

4RUB is a Protein complex structure of sequences from Nicotiana tabacum. Full crystallographic information is available from OCA.

Reference

A crystal form of ribulose-1,5-bisphosphate carboxylase/oxygenase from Nicotiana tabacum in the activated state., Suh SW, Cascio D, Chapman MS, Eisenberg D, J Mol Biol. 1987 Sep 20;197(2):363-5. PMID:3681999 Page seeded by OCA on Sun May 4 22:29:08 2008

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