5icd

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[[Image:5icd.gif|left|200px]]
[[Image:5icd.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 5icd |SIZE=350|CAPTION= <scene name='initialview01'>5icd</scene>, resolution 2.5&Aring;
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The line below this paragraph, containing "STRUCTURE_5icd", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=ICT:ISOCITRIC+ACID'>ICT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Isocitrate_dehydrogenase_(NADP(+)) Isocitrate dehydrogenase (NADP(+))], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.42 1.1.1.42] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_5icd| PDB=5icd | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5icd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5icd OCA], [http://www.ebi.ac.uk/pdbsum/5icd PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=5icd RCSB]</span>
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}}
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'''REGULATION OF AN ENZYME BY PHOSPHORYLATION AT THE ACTIVE SITE'''
'''REGULATION OF AN ENZYME BY PHOSPHORYLATION AT THE ACTIVE SITE'''
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Regulation of an enzyme by phosphorylation at the active site., Hurley JH, Dean AM, Sohl JL, Koshland DE Jr, Stroud RM, Science. 1990 Aug 31;249(4972):1012-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/2204109 2204109]
Regulation of an enzyme by phosphorylation at the active site., Hurley JH, Dean AM, Sohl JL, Koshland DE Jr, Stroud RM, Science. 1990 Aug 31;249(4972):1012-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/2204109 2204109]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Isocitrate dehydrogenase (NADP(+))]]
 
[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Dean, A M.]]
[[Category: Dean, A M.]]
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[[Category: Sohl, J L.]]
[[Category: Sohl, J L.]]
[[Category: Stroud, R M.]]
[[Category: Stroud, R M.]]
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[[Category: oxidoreductase (nad(a)-choh(d))]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 22:35:20 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:41:42 2008''
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Revision as of 19:35, 4 May 2008

Image:5icd.gif

Template:STRUCTURE 5icd

REGULATION OF AN ENZYME BY PHOSPHORYLATION AT THE ACTIVE SITE


Overview

The isocitrate dehydrogenase of Escherichia coli is an example of a ubiquitous class of enzymes that are regulated by covalent modification. In the three-dimensional structure of the enzyme-substrate complex, isocitrate forms a hydrogen bond with Ser113, the site of regulatory phosphorylation. The structures of Asp113 and Glu113 mutants, which mimic the inactivation of the enzyme by phosphorylation, show minimal conformational changes from wild type, as in the phosphorylated enzyme. Calculations based on observed structures suggest that the change in electrostatic potential when a negative charge is introduced either by phosporylation or site-directed mutagenesis is sufficient to inactivate the enzyme. Thus, direct interaction at a ligand binding site is an alternative mechanism to induced conformational changes from an allosteric site in the regulation of protein activity by phosphorylation.

About this Structure

5ICD is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Regulation of an enzyme by phosphorylation at the active site., Hurley JH, Dean AM, Sohl JL, Koshland DE Jr, Stroud RM, Science. 1990 Aug 31;249(4972):1012-6. PMID:2204109 Page seeded by OCA on Sun May 4 22:35:20 2008

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