6tli

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[[Image:6tli.gif|left|200px]]
[[Image:6tli.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 6tli |SIZE=350|CAPTION= <scene name='initialview01'>6tli</scene>, resolution 2.10&Aring;
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The line below this paragraph, containing "STRUCTURE_6tli", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Thermolysin Thermolysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.27 3.4.24.27] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_6tli| PDB=6tli | SCENE= }}
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|RELATEDENTRY=[[1tlx|1TLX]], [[2tlx|2TLX]], [[1tli|1TLI]], [[2tli|2TLI]], [[3tli|3TLI]], [[4tli|4TLI]], [[5tli|5TLI]], [[7tli|7TLI]], [[8tli|8TLI]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6tli FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tli OCA], [http://www.ebi.ac.uk/pdbsum/6tli PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=6tli RCSB]</span>
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}}
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'''THERMOLYSIN (60% ISOPROPANOL SOAKED CRYSTALS)'''
'''THERMOLYSIN (60% ISOPROPANOL SOAKED CRYSTALS)'''
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[[Category: Groom, C R.]]
[[Category: Groom, C R.]]
[[Category: Hubbard, R E.]]
[[Category: Hubbard, R E.]]
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[[Category: hydrolase]]
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[[Category: Hydrolase]]
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[[Category: metalloproteinase]]
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[[Category: Metalloproteinase]]
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[[Category: organic solvent]]
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[[Category: Organic solvent]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 22:43:06 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:43:42 2008''
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Revision as of 19:43, 4 May 2008

Image:6tli.gif

Template:STRUCTURE 6tli

THERMOLYSIN (60% ISOPROPANOL SOAKED CRYSTALS)


Overview

Multiple-solvent crystal structure determination (MSCS) allows the position and orientation of bound solvent fragments to be identified by determining the structure of protein crystals soaked in organic solvents. We have extended this technique by the determination of high-resolution crystal structures of thermolysin (TLN), generated from crystals soaked in 2% to 100% isopropanol. The procedure causes only minor changes to the conformation of the protein, and an increasing number of isopropanol interaction sites could be identified as the solvent concentration is increased. Isopropanol occupies all four of the main subsites in the active site, although this was only observed at very high concentrations of isopropanol for three of the four subsites. Analysis of the isopropanol positions shows little correlation with interaction energy computed using a molecular mechanics force field, but the experimentally determined positions of isopropanol are consistent with the structures of known protein-ligand complexes of TLN.

About this Structure

6TLI is a Single protein structure of sequence from Bacillus thermoproteolyticus. Full crystallographic information is available from OCA.

Reference

Locating interaction sites on proteins: the crystal structure of thermolysin soaked in 2% to 100% isopropanol., English AC, Done SH, Caves LS, Groom CR, Hubbard RE, Proteins. 1999 Dec 1;37(4):628-40. PMID:10651278 Page seeded by OCA on Sun May 4 22:43:06 2008

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OCA

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