7msf

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[[Image:7msf.gif|left|200px]]
[[Image:7msf.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 7msf |SIZE=350|CAPTION= <scene name='initialview01'>7msf</scene>, resolution 2.8&Aring;
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The line below this paragraph, containing "STRUCTURE_7msf", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=A:ADENOSINE-5&#39;-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5&#39;-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5&#39;-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=U:URIDINE-5&#39;-MONOPHOSPHATE'>U</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE= COAT PROTEIN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=12022 Enterobacterio phage MS2])
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-->
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|DOMAIN=
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{{STRUCTURE_7msf| PDB=7msf | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=7msf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7msf OCA], [http://www.ebi.ac.uk/pdbsum/7msf PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=7msf RCSB]</span>
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}}
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'''MS2 PROTEIN CAPSID/RNA COMPLEX'''
'''MS2 PROTEIN CAPSID/RNA COMPLEX'''
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[[Category: Stockley, P G.]]
[[Category: Stockley, P G.]]
[[Category: Stonehouse, N J.]]
[[Category: Stonehouse, N J.]]
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[[Category: bacteriophage ms2]]
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[[Category: Bacteriophage ms2]]
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[[Category: ccapsid protein ms2/rna aptamer complex]]
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[[Category: Ccapsid protein ms2/rna aptamer complex]]
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[[Category: icosahedral virus]]
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[[Category: Icosahedral virus]]
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[[Category: rna stem loop]]
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[[Category: Rna stem loop]]
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[[Category: rna-protein complex]]
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[[Category: Rna-protein complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 22:46:42 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:44:31 2008''
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Revision as of 19:46, 4 May 2008

Image:7msf.gif

Template:STRUCTURE 7msf

MS2 PROTEIN CAPSID/RNA COMPLEX


Overview

We have determined the crystal structures, at 2.8 A resolution, of two different RNA aptamers, each bound to MS2 coat protein. One of the aptamers contains a non-Watson-Crick base pair, while the other is missing one of the unpaired adenines that make sequence-specific contacts in the wild-type complex. Despite these differences, the RNA aptamers bind in the same location on the protein as the wild-type translational operator. Comparison of these new structures with other MS2-RNA complexes allows us to refine further the definition of the minimal recognition elements and suggests a possible application of the MS2 system for routine structure determination of small nucleic acid motifs.

About this Structure

7MSF is a Single protein structure of sequence from Enterobacterio phage ms2. Full crystallographic information is available from OCA.

Reference

Crystal structures of a series of RNA aptamers complexed to the same protein target., Rowsell S, Stonehouse NJ, Convery MA, Adams CJ, Ellington AD, Hirao I, Peabody DS, Stockley PG, Phillips SE, Nat Struct Biol. 1998 Nov;5(11):970-5. PMID:9808042 Page seeded by OCA on Sun May 4 22:46:42 2008

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