7rsa

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[[Image:7rsa.gif|left|200px]]
[[Image:7rsa.gif|left|200px]]
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{{Structure
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|PDB= 7rsa |SIZE=350|CAPTION= <scene name='initialview01'>7rsa</scene>, resolution 1.26&Aring;
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The line below this paragraph, containing "STRUCTURE_7rsa", creates the "Structure Box" on the page.
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|SITE=
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|LIGAND= <scene name='pdbligand=DOD:DEUTERATED+WATER'>DOD</scene>, <scene name='pdbligand=TBU:TERTIARY-BUTYL+ALCOHOL'>TBU</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] </span>
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{{STRUCTURE_7rsa| PDB=7rsa | SCENE= }}
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=7rsa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7rsa OCA], [http://www.ebi.ac.uk/pdbsum/7rsa PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=7rsa RCSB]</span>
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'''STRUCTURE OF PHOSPHATE-FREE RIBONUCLEASE A REFINED AT 1.26 ANGSTROMS'''
'''STRUCTURE OF PHOSPHATE-FREE RIBONUCLEASE A REFINED AT 1.26 ANGSTROMS'''
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[[Category: Gilliland, G L.]]
[[Category: Gilliland, G L.]]
[[Category: Wlodawer, A.]]
[[Category: Wlodawer, A.]]
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[[Category: hydrolase (phosphoric diester)]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 22:47:35 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:44:44 2008''
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Revision as of 19:47, 4 May 2008

Image:7rsa.gif


PDB ID 7rsa

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7rsa, resolution 1.26Å ()
Ligands: ,
Activity: Pancreatic ribonuclease, with EC number 3.1.27.5
Resources: FirstGlance, OCA, RCSB, PDBsum
Coordinates: save as pdb, mmCIF, xml



STRUCTURE OF PHOSPHATE-FREE RIBONUCLEASE A REFINED AT 1.26 ANGSTROMS


Overview

The structure of phosphate-free bovine ribonuclease A has been refined at 1.26-A resolution by a restrained least-squares procedure to a final R factor of 0.15. X-ray diffraction data were collected with an electronic position-sensitive detector. The final model consists of all atoms in the polypeptide chain including hydrogens, 188 water sites with full or partial occupancy, and a single molecule of 2-methyl-2-propanol. Thirteen side chains were modeled with two alternate conformations. Major changes to the active site include the addition of two waters in the phosphate-binding pocket, disordering of Gln-11, and tilting of the imidazole ring of His-119. The structure of the protein and of the associated solvent was extensively compared with three other high-resolution, refined structures of this enzyme.

About this Structure

7RSA is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.

Reference

Structure of phosphate-free ribonuclease A refined at 1.26 A., Wlodawer A, Svensson LA, Sjolin L, Gilliland GL, Biochemistry. 1988 Apr 19;27(8):2705-17. PMID:3401445 Page seeded by OCA on Sun May 4 22:47:35 2008

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