8mht

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[[Image:8mht.gif|left|200px]]
[[Image:8mht.gif|left|200px]]
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{{Structure
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|PDB= 8mht |SIZE=350|CAPTION= <scene name='initialview01'>8mht</scene>, resolution 2.76&Aring;
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The line below this paragraph, containing "STRUCTURE_8mht", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=DU:2&#39;-DEOXYURIDINE-5&#39;-MONOPHOSPHATE'>DU</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Deleted_entry Deleted entry], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.73 2.1.1.73] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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|DOMAIN=
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{{STRUCTURE_8mht| PDB=8mht | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=8mht FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8mht OCA], [http://www.ebi.ac.uk/pdbsum/8mht PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=8mht RCSB]</span>
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}}
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'''CYTOSINE-SPECIFIC METHYLTRANSFERASE HHAI/DNA COMPLEX'''
'''CYTOSINE-SPECIFIC METHYLTRANSFERASE HHAI/DNA COMPLEX'''
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[[Category: Horton, J R.]]
[[Category: Horton, J R.]]
[[Category: Roberts, R J.]]
[[Category: Roberts, R J.]]
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[[Category: complex (methyltransferase/ dna)]]
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[[Category: Methyltransferase]]
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[[Category: methyltransferase]]
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[[Category: Restriction system]]
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[[Category: restriction system]]
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[[Category: Transferase]]
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[[Category: transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 22:51:14 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:45:34 2008''
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Revision as of 19:51, 4 May 2008

Image:8mht.gif

Template:STRUCTURE 8mht

CYTOSINE-SPECIFIC METHYLTRANSFERASE HHAI/DNA COMPLEX


Overview

Three structures have been determined for complexes between HhaI methyltransferase (M.HhaI) and oligonucleotides containing a G:A, G:U or G:AP (AP = abasic or apurinic/apyrimidinic) mismatch at the target base pair. The mismatched adenine, uracil and abasic site are all flipped out of the DNA helix and located in the enzyme's active-site pocket, adopting the same conformation as in the flipped-out normal substrate. These results, particularly the flipped-out abasic deoxyribose sugar, provide insight into the mechanism of base flipping. If the process involves the protein pushing the base out of the helix, then the push must take place not on the base, but rather on the sugar-phosphate backbone. Thus rotation of the DNA backbone is probably the key to base flipping.

About this Structure

8MHT is a Single protein structure of sequence from Haemophilus haemolyticus. Full crystallographic information is available from OCA.

Reference

Structures of HhaI methyltransferase complexed with substrates containing mismatches at the target base., O'Gara M, Horton JR, Roberts RJ, Cheng X, Nat Struct Biol. 1998 Oct;5(10):872-7. PMID:9783745 Page seeded by OCA on Sun May 4 22:51:14 2008

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OCA

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