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spinqualitylabelsall models 1b54, resolution 2.1Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

CRYSTAL STRUCTURE OF A YEAST HYPOTHETICAL PROTEIN-A STRUCTURE FROM BNL'S HUMAN PROTEOME PROJECT

Overview

Yeast hypothetical protein YBL036C (SWISS-PROT P38197), initially thought, to be a member of an 11-protein family, was selected for crystal structure, determination since no structural or functional information was available., The structure has been determined independently by MIR and MAD methods to, 2.0 A resolution. The MAD structure was determined largely through, automated model building. The protein folds as a TIM barrel beginning with, a long N-terminal helix, in contrast to the classic triose phosphate, isomerase (TIM) structure, which begins with a beta-strand. A cofactor, pyridoxal 5'-phosphate, is covalently bound near the C-terminal end of the, barrel, the usual active site in TIM-barrel folds. A single-domain, monomeric molecule, this yeast protein resembles the N-terminal domain of, alanine racemase or ornithine decarboxylase, both of which are two-domain, dimeric proteins. The yeast protein has been shown to have amino-acid, racemase activity. Although selected as a member of a protein family, having no obvious relationship to proteins of known structure, the protein, fold turned out to be a well known and widely distributed fold. This, points to the need for a more comprehensive base of structural information, and better structure-modeling tools before the goal of structure, prediction from amino-acid sequences can be realised. In this case, similarity to a known structure allowed inferences to be made about the, structure and function of a widely distributed protein family.

About this Structure

1B54 is a Single protein structure of sequence from Saccharomyces cerevisiae with PLP as ligand. Full crystallographic information is available from OCA.

Reference

Structure of a yeast hypothetical protein selected by a structural genomics approach., Eswaramoorthy S, Gerchman S, Graziano V, Kycia H, Studier FW, Swaminathan S, Acta Crystallogr D Biol Crystallogr. 2003 Jan;59(Pt 1):127-35. Epub 2002, Dec 19. PMID:12499548

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