1b8d

From Proteopedia

(Difference between revisions)
Jump to: navigation, search

OCA (Talk | contribs)
(New page: 200px<br /><applet load="1b8d" size="450" color="white" frame="true" align="right" spinBox="true" caption="1b8d, resolution 1.90&Aring;" /> '''CRYSTAL STRUCTURE OF...)
Next diff →

Revision as of 09:19, 20 November 2007

Image:1b8d.gif

1b8d, resolution 1.90Å

Drag the structure with the mouse to rotate

CRYSTAL STRUCTURE OF A PHYCOUROBILIN-CONTAINING PHYCOERYTHRIN

Overview

The structure of R-phycoerythrin (R-PE) from the red alga Griffithsia, monilis was solved at 1.90-A resolution by molecular replacement, using, the atomic coordinates of cyanobacterial phycocyanin from Fremyella, diplosiphon as a model. The crystallographic R factor for the final model, is 17.5% (Rfree 22.7%) for reflections in the range 100-1.90 A. The model, consists of an (alphabeta)2 dimer with an internal noncrystallographic, dyad and a fragment of the gamma-polypeptide. The alpha-polypeptide (164, amino acid residues) has two covalently bound phycoerythrobilins at, positions alpha82 and alpha139. The beta-polypeptide (177 residues) has, two phycoerythrobilins bound to residues beta82 and beta158 and one, phycourobilin covalently attached to rings A and D at residues beta50 and, beta61, respectively. The electron density of the gamma-polypeptide is, mostly averaged out by threefold crystallographic symmetry, but a, dipeptide (Gly-Tyr) and one single Tyr could be modeled. These two, tyrosine residues of the gamma-polypeptide are in close proximity to the, phycoerythrobilins at position beta82 of two symmetry-related, beta-polypeptides and are related by the same noncrystallographic dyad as, the (alphabeta)2 dimer. Possible energy transfer pathways are discussed, briefly.

About this Structure

1B8D is a Protein complex structure of sequences from Griffithsia monilis with PEB and PUB as ligands. Full crystallographic information is available from OCA.

Reference

Crystal structure of a phycourobilin-containing phycoerythrin at 1.90-A resolution., Ritter S, Hiller RG, Wrench PM, Welte W, Diederichs K, J Struct Biol. 1999 Jun 15;126(2):86-97. PMID:10388620

Page seeded by OCA on Tue Nov 20 11:26:48 2007

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1b8d"
Personal tools