1rp0
From Proteopedia
| Line 11: | Line 11: | ||
'''Crystal Structure of Thi1 protein from Arabidopsis thaliana''' | '''Crystal Structure of Thi1 protein from Arabidopsis thaliana''' | ||
| + | |||
| + | ==Overview== | ||
| + | Thiamin pyrophosphate is an essential coenzyme in all organisms that depend on fermentation, respiration or photosynthesis to produce ATP. It is synthesized through two independent biosynthetic routes: one for the synthesis of 2-methyl-4-amino-5-hydroxymethylpyrimidine pyrophosphate (pyrimidine moiety) and another for the synthesis of 4-methyl-5-(beta-hydroxyethyl) thiazole phosphate (thiazole moiety). Herein, we will describe the three-dimensional structure of THI1 protein from Arabidopsis thaliana determined by single wavelength anomalous diffraction to 1.6A resolution. The protein was produced using heterologous expression in bacteria, unexpectedly bound to 2-carboxylate-4-methyl-5-beta-(ethyl adenosine 5-diphosphate) thiazole, a potential intermediate of the thiazole biosynthesis in Eukaryotes. THI1 has a topology similar to dinucleotide binding domains and although details concerning its function are unknown, this work provides new clues about the thiazole biosynthesis in Eukaryotes. | ||
==About this Structure== | ==About this Structure== | ||
1RP0 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RP0 OCA]. | 1RP0 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RP0 OCA]. | ||
| + | |||
| + | ==Reference== | ||
| + | Structure of the thiazole biosynthetic enzyme THI1 from Arabidopsis thaliana., Godoi PH, Galhardo RS, Luche DD, Van Sluys MA, Menck CF, Oliva G, J Biol Chem. 2006 Oct 13;281(41):30957-66. Epub 2006 Aug 15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16912043 16912043] | ||
[[Category: Arabidopsis thaliana]] | [[Category: Arabidopsis thaliana]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 20: | Line 26: | ||
[[Category: Oliva, G.]] | [[Category: Oliva, G.]] | ||
[[Category: Sluys, M A.Van.]] | [[Category: Sluys, M A.Van.]] | ||
| + | [[Category: Biosynthetic protein]] | ||
[[Category: Protein ligand complex]] | [[Category: Protein ligand complex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu May 22 22:16:23 2008'' |
Revision as of 19:16, 22 May 2008
Crystal Structure of Thi1 protein from Arabidopsis thaliana
Overview
Thiamin pyrophosphate is an essential coenzyme in all organisms that depend on fermentation, respiration or photosynthesis to produce ATP. It is synthesized through two independent biosynthetic routes: one for the synthesis of 2-methyl-4-amino-5-hydroxymethylpyrimidine pyrophosphate (pyrimidine moiety) and another for the synthesis of 4-methyl-5-(beta-hydroxyethyl) thiazole phosphate (thiazole moiety). Herein, we will describe the three-dimensional structure of THI1 protein from Arabidopsis thaliana determined by single wavelength anomalous diffraction to 1.6A resolution. The protein was produced using heterologous expression in bacteria, unexpectedly bound to 2-carboxylate-4-methyl-5-beta-(ethyl adenosine 5-diphosphate) thiazole, a potential intermediate of the thiazole biosynthesis in Eukaryotes. THI1 has a topology similar to dinucleotide binding domains and although details concerning its function are unknown, this work provides new clues about the thiazole biosynthesis in Eukaryotes.
About this Structure
1RP0 is a Single protein structure of sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA.
Reference
Structure of the thiazole biosynthetic enzyme THI1 from Arabidopsis thaliana., Godoi PH, Galhardo RS, Luche DD, Van Sluys MA, Menck CF, Oliva G, J Biol Chem. 2006 Oct 13;281(41):30957-66. Epub 2006 Aug 15. PMID:16912043 Page seeded by OCA on Thu May 22 22:16:23 2008
