3bs9
From Proteopedia
| Line 11: | Line 11: | ||
'''X-ray structure of human TIA-1 RRM2''' | '''X-ray structure of human TIA-1 RRM2''' | ||
| + | |||
| + | ==Overview== | ||
| + | T-cell-restricted intracellular antigen-1 (TIA-1) regulates alternative pre-mRNA splicing in the nucleus, and mRNA translation in the cytoplasm, by recognizing uridine-rich sequences of RNAs. As a step towards understanding RNA recognition by this regulatory factor, the X-ray structure of the central RNA recognition motif (RRM2) of human TIA-1 is presented at 1.95A resolution. Comparison with structurally homologous RRM-RNA complexes identifies residues at the RNA interfaces that are conserved in TIA-1-RRM2. The versatile capability of RNP motifs to interact with either proteins or RNA is reinforced by symmetry-related protein-protein interactions mediated by the RNP motifs of TIA-1-RRM2. Importantly, the TIA-1-RRM2 structure reveals the locations of mutations responsible for inhibiting nuclear import. In contrast with previous assumptions, the mutated residues are buried within the hydrophobic interior of the domain, where they would be likely to destabilize the RRM fold rather than directly inhibit RNA binding. | ||
==About this Structure== | ==About this Structure== | ||
3BS9 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BS9 OCA]. | 3BS9 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BS9 OCA]. | ||
| + | |||
| + | ==Reference== | ||
| + | Structure of the central RNA recognition motif of human TIA-1 at 1.95A resolution., Kumar AO, Swenson MC, Benning MM, Kielkopf CL, Biochem Biophys Res Commun. 2008 Mar 21;367(4):813-9. Epub 2008 Jan 15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18201561 18201561] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 27: | Line 33: | ||
[[Category: Rna-binding]] | [[Category: Rna-binding]] | ||
[[Category: Rrm]] | [[Category: Rrm]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu May 22 22:35:58 2008'' |
Revision as of 19:36, 22 May 2008
X-ray structure of human TIA-1 RRM2
Overview
T-cell-restricted intracellular antigen-1 (TIA-1) regulates alternative pre-mRNA splicing in the nucleus, and mRNA translation in the cytoplasm, by recognizing uridine-rich sequences of RNAs. As a step towards understanding RNA recognition by this regulatory factor, the X-ray structure of the central RNA recognition motif (RRM2) of human TIA-1 is presented at 1.95A resolution. Comparison with structurally homologous RRM-RNA complexes identifies residues at the RNA interfaces that are conserved in TIA-1-RRM2. The versatile capability of RNP motifs to interact with either proteins or RNA is reinforced by symmetry-related protein-protein interactions mediated by the RNP motifs of TIA-1-RRM2. Importantly, the TIA-1-RRM2 structure reveals the locations of mutations responsible for inhibiting nuclear import. In contrast with previous assumptions, the mutated residues are buried within the hydrophobic interior of the domain, where they would be likely to destabilize the RRM fold rather than directly inhibit RNA binding.
About this Structure
3BS9 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structure of the central RNA recognition motif of human TIA-1 at 1.95A resolution., Kumar AO, Swenson MC, Benning MM, Kielkopf CL, Biochem Biophys Res Commun. 2008 Mar 21;367(4):813-9. Epub 2008 Jan 15. PMID:18201561 Page seeded by OCA on Thu May 22 22:35:58 2008
