1bjf
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(New page: 200px<br /><applet load="1bjf" size="450" color="white" frame="true" align="right" spinBox="true" caption="1bjf, resolution 2.4Å" /> '''CRYSTAL STRUCTURE OF ...)
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Revision as of 09:34, 20 November 2007
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CRYSTAL STRUCTURE OF RECOMBINANT BOVINE NEUROCALCIN DELTA AT 2.4 ANGSTROMS
Overview
The crystal structure of calcium-bound unmyristoylated bovine neurocalcin, from Escherichia coli has been determined at 2.4 A resolution. The, three-dimensional structure reveals a highly compact structure consisting, of: (i) two pairs of calcium-binding EF-hands (EF1-EF2 and EF3-EF4); (ii), a calcium ion bound at EF2, EF3 and EF4 sites; and (iii) an EF1-hand that, is disabled from calcium-binding due to a Cys-Pro sequence in the, Ca2+-binding loop. The crystal structure of neurocalcin resembles, photoreceptor recoverin in overall topology, however its EF2- and, EF4-hands differ. Recently, neurocalcin in the calcium-bound state has, been shown to stimulate mammalian rod outer segment membrane guanylate, cyclase. A possible site for cyclase activity based on the, three-dimensional structure is discussed.
About this Structure
1BJF is a Single protein structure of sequence from Escherichia coli with CA as ligand. Full crystallographic information is available from OCA.
Reference
Crystal structure of recombinant bovine neurocalcin., Vijay-Kumar S, Kumar VD, Nat Struct Biol. 1999 Jan;6(1):80-8. PMID:9886296
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