1bk7
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(New page: 200px<br /><applet load="1bk7" size="450" color="white" frame="true" align="right" spinBox="true" caption="1bk7, resolution 1.75Å" /> '''RIBONUCLEASE MC1 FRO...)
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Revision as of 09:35, 20 November 2007
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RIBONUCLEASE MC1 FROM THE SEEDS OF BITTER GOURD
Overview
The ribonuclease MC1 (RNase MC1) from seeds of bitter gourd (Momordica, charantia) consists of 190 amino acid residues with four disulfide bridges, and belongs to the RNase T(2) family, including fungal RNases typified by, RNase Rh from Rhizopus niveus and RNase T(2) from Aspergillus oryzae. The, crystal structure of RNase MC1 has been determined at 1.75 A resolution, with an R-factor of 19.7% using the single isomorphous replacement method., RNase MC1 structurally belongs to the (alpha+beta) class of proteins, having ten helices (six alpha-helices and four 3(10)-helices) and eight, beta-strands. When the structures of RNase MC1 and RNase Rh are, superposed, the close agreement between the alpha-carbon positions for the, total structure is obvious: the root mean square deviations calculated, only for structurally related 151 alpha-carbon atoms of RNase MC1 and, RNase Rh molecules was 1.76 A. Furthermore, the conformation of the, catalytic residues His-46, Glu-105, and His-109 in RNase Rh can be easily, superposed with that of the possible catalytic residues His-34, Glu-84, and His-88 in RNase MC1. This observation strongly indicates that RNase, MC1 from a plant origin catalyzes RNA degradation in a similar manner as, fungal RNases.
About this Structure
1BK7 is a Single protein structure of sequence from Momordica charantia. Active as Ribonuclease T(2), with EC number 3.1.27.1 Full crystallographic information is available from OCA.
Reference
Crystal structure of a ribonuclease from the seeds of bitter gourd (Momordica charantia) at 1.75 A resolution., Nakagawa A, Tanaka I, Sakai R, Nakashima T, Funatsu G, Kimura M, Biochim Biophys Acta. 1999 Aug 17;1433(1-2):253-60. PMID:10446375
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