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1bm8
From Proteopedia
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(New page: 200px<br /><applet load="1bm8" size="450" color="white" frame="true" align="right" spinBox="true" caption="1bm8, resolution 1.71Å" /> '''DNA-BINDING DOMAIN O...)
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Revision as of 09:38, 20 November 2007
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DNA-BINDING DOMAIN OF MBP1
Overview
BACKGROUND: During the cell cycle, cells progress through four distinct, phases, G1, S, G2 and M; transcriptional controls play an important role, at the transition between these phases. MCB-binding factor (MBF), a, transcription factor from budding yeast, binds to the so-called MCB (MluI, cell-cycle box) elements found in the promoters of many DNA synthesis, genes, and activates the transcription of those at the G1-->S phase, transition. MBF is comprised of two proteins, Mbp1 and Swi6. RESULTS: The, three-dimensional structure of the N-terminal DNA-binding domain of Mbp1, has been determined by multiwavelength anomalous diffraction from crystals, of the selenomethionyl variant of the protein. The structure is composed, of a six-stranded beta sheet interspersed with two pairs of alpha helices., The most conserved core region among Mbp1-related transcription factors, folds into a central helix-turn-helix motif with a short N-terminal beta, strand and a C-terminal beta hairpin. CONCLUSIONS: Despite little sequence, similarity, the structure within the core region of the Mbp1 N-terminal, domain exhibits a similar fold to that of the DNA-binding domains of other, proteins, such as hepatocyte nuclear factor-3gamma and histone H5 from, eukaryotes, and the prokaryotic catabolite gene activator. However, the, structure outside the core region defines Mbp1 as a larger entity with, substructures that stabilize and display the helix-turn-helix motif.
About this Structure
1BM8 is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Crystal structure of the DNA-binding domain of Mbp1, a transcription factor important in cell-cycle control of DNA synthesis., Xu RM, Koch C, Liu Y, Horton JR, Knapp D, Nasmyth K, Cheng X, Structure. 1997 Mar 15;5(3):349-58. PMID:9083114
Page seeded by OCA on Tue Nov 20 11:45:14 2007
