1bsj
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(New page: 200px<br /><applet load="1bsj" size="450" color="white" frame="true" align="right" spinBox="true" caption="1bsj, resolution 3.0Å" /> '''COBALT DEFORMYLASE IN...)
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Revision as of 09:46, 20 November 2007
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COBALT DEFORMYLASE INHIBITOR COMPLEX FROM E.COLI
Overview
While protein synthesis in bacteria begins with a formylated methionine, the formyl group of the nascent polypeptide is removed by peptide, deformylase. Since eukaryotic protein synthesis does not involve, formylation and deformylation at the N-terminus, there has been increasing, interest in peptide deformylase as a potential target for antibacterial, chemotherapy. Toward this end and to aid in the design of effective, antibiotics targeting peptide deformylase, the structures of the, protein-inhibitor complexes of both the cobalt and the zinc containing, Escherichia coli peptide deformylase bound to the transition-state, analogue, (S)-2-O-(H-phosphonoxy)-L-caproyl-L-leucyl-p-nitroanilide, (PCLNA), have been determined. The proteins for both deformylase-inhibitor, complexes show basically the same fold as for the native enzyme. The PCLNA, inhibitor adopts an extended conformation and fits nicely into a, hydrophobic cavity located near the metal site. On the basis of these, structures, guidelines for the design of high-affinity deformylase, inhibitors are suggested. As our results show that the protein residues, which interact with the PCLNA inhibitor are conserved over a wide variety, of species, we suggest that antibiotics targeting deformylase could have, wide applicability.
About this Structure
1BSJ is a Single protein structure of sequence from Escherichia coli with CO, PO4 and MLN as ligands. Active as N-formylmethionylaminoacyl-tRNA deformylase, with EC number 3.5.1.27 Full crystallographic information is available from OCA.
Reference
Structural basis for the design of antibiotics targeting peptide deformylase., Hao B, Gong W, Rajagopalan PT, Zhou Y, Pei D, Chan MK, Biochemistry. 1999 Apr 13;38(15):4712-9. PMID:10200158
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