Sandbox 1

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-	You can take a close look at the chromophore of '''GFP''' in the PDB entry [[1ema]]<applet load='1ema' size='300' frame='true' align='right' caption='Insert caption here' />. The backbone of the entire protein is shown here on the left. The protein chain forms a cylindrical can (shown in blue), with one portion of the strand threading straight through the middle (shown in green). The chromophore is found <scene name='Sandbox_1/Scene1/1'>right in the middle</scene> of the can , totally shielded from the surrounding environment.This shielding is essential for the fluorescence. The jostling water molecules would normally rob the chromophore of its energy once it absorbs a photon. But inside the protein, it is protected, releasing the energy instead as a slightly less energetic photon of light. The chromophore (shown in the close-up on the right) forms spontaneously from three amino acids in the protein chain: a glycine, a tyrosine and a threonine (or serine). Notice how the glycine and the threonine have formed a new bond, creating an unusual five-membered ring.	+

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Revision as of 12:43, 31 May 2008