3c2j
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Revision as of 06:54, 4 June 2008
Crystal structure analysis of trioxacarcin A covalently bound to d(AACCGGTT)
Overview
We report a crystal structure that shows an antibiotic that extracts a nucleobase from a DNA molecule 'caught in the act' after forming a covalent bond but before departing with the base. The structure of trioxacarcin A covalently bound to double-stranded d(AACCGGTT) was determined to 1.78 A resolution by MAD phasing employing brominated oligonucleotides. The DNA-drug complex has a unique structure that combines alkylation (at the N7 position of a guanine), intercalation (on the 3'-side of the alkylated guanine), and base flip-out. An antibiotic-induced flipping-out of a single, nonterminal nucleobase from a DNA duplex was observed for the first time in a crystal structure.
About this Structure
3C2J is a Single protein structure. Full crystallographic information is available from OCA.
Reference
Crystal structure of trioxacarcin A covalently bound to DNA., Pfoh R, Laatsch H, Sheldrick GM, Nucleic Acids Res. 2008 May 3;. PMID:18453630 Page seeded by OCA on Wed Jun 4 09:54:35 2008
