2qs6
From Proteopedia
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'''Structure of a Hoogsteen antiparallel duplex with extra-helical thymines''' | '''Structure of a Hoogsteen antiparallel duplex with extra-helical thymines''' | ||
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| + | ==Overview== | ||
| + | We present the crystal structure of the DNA duplex formed by d(ATATATCT). The crystals contain seven stacked antiparallel duplexes in the asymmetric unit with A.T Hoogsteen base pairs. The terminal CT sequences bend over so that the thymines enter the minor groove and form a hydrogen bond with thymine 2 of the complementary strand in the Hoogsteen duplex. Cytosines occupy extra-helical positions; they contribute to the crystal lattice through various kinds of interactions, including a unique CAA triplet. The presence of thymine in the minor groove apparently contributes to the stability of the DNA duplex in the Hoogsteen conformation. These observations open the way toward finding under what conditions the Hoogsteen duplex may be stabilized in vivo. The present crystal structure also confirms the tendency of A.T-rich oligonucleotides to crystallize as long helical stacks of duplexes. | ||
==About this Structure== | ==About this Structure== | ||
2QS6 is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QS6 OCA]. | 2QS6 is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QS6 OCA]. | ||
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| + | ==Reference== | ||
| + | Stabilization by extra-helical thymines of a DNA duplex with Hoogsteen base pairs., Pous J, Urpi L, Subirana JA, Gouyette C, Navaza J, Campos JL, J Am Chem Soc. 2008 May 28;130(21):6755-60. Epub 2008 May 1. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18447354 18447354] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Campos, J L.]] | [[Category: Campos, J L.]] | ||
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[[Category: Subirana, J A.]] | [[Category: Subirana, J A.]] | ||
[[Category: Urpi, L.]] | [[Category: Urpi, L.]] | ||
| + | [[Category: Dna]] | ||
[[Category: Hoogsteen]] | [[Category: Hoogsteen]] | ||
| - | [[Category: Oligonucleotide | + | [[Category: Oligonucleotide]] |
[[Category: Thymine]] | [[Category: Thymine]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 4 09:58:45 2008'' |
Revision as of 06:58, 4 June 2008
Structure of a Hoogsteen antiparallel duplex with extra-helical thymines
Overview
We present the crystal structure of the DNA duplex formed by d(ATATATCT). The crystals contain seven stacked antiparallel duplexes in the asymmetric unit with A.T Hoogsteen base pairs. The terminal CT sequences bend over so that the thymines enter the minor groove and form a hydrogen bond with thymine 2 of the complementary strand in the Hoogsteen duplex. Cytosines occupy extra-helical positions; they contribute to the crystal lattice through various kinds of interactions, including a unique CAA triplet. The presence of thymine in the minor groove apparently contributes to the stability of the DNA duplex in the Hoogsteen conformation. These observations open the way toward finding under what conditions the Hoogsteen duplex may be stabilized in vivo. The present crystal structure also confirms the tendency of A.T-rich oligonucleotides to crystallize as long helical stacks of duplexes.
About this Structure
2QS6 is a Single protein structure. Full crystallographic information is available from OCA.
Reference
Stabilization by extra-helical thymines of a DNA duplex with Hoogsteen base pairs., Pous J, Urpi L, Subirana JA, Gouyette C, Navaza J, Campos JL, J Am Chem Soc. 2008 May 28;130(21):6755-60. Epub 2008 May 1. PMID:18447354 Page seeded by OCA on Wed Jun 4 09:58:45 2008
Categories: Single protein | Campos, J L. | Gouyette, C. | Navaza, J. | Pous, J. | Subirana, J A. | Urpi, L. | Dna | Hoogsteen | Oligonucleotide | Thymine
