2o20
From Proteopedia
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'''Crystal structure of transcription regulator CcpA of Lactococcus lactis''' | '''Crystal structure of transcription regulator CcpA of Lactococcus lactis''' | ||
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| + | ==Overview== | ||
| + | Catabolite control protein A (CcpA) functions as master transcriptional regulator of carbon catabolism in Firmicutes. It belongs to the family of bacterial repressor/regulator proteins. Here, the crystal structure of the 76 kDa homodimeric CcpA protein from Lactococcus lactis subsp. lactis IL1403 is presented at 1.9 A resolution in the absence of cognate DNA. The phases were derived by molecular replacement and the structure was refined to crystallographic R and R(free) factors of 0.177 and 0.211, respectively. The presence of a sulfate molecule in the direct vicinity of a putative effector-binding site in the monomer allowed the derivation of a model for the possible binding of small organic effector molecules. | ||
==About this Structure== | ==About this Structure== | ||
2O20 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Lactococcus_lactis Lactococcus lactis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O20 OCA]. | 2O20 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Lactococcus_lactis Lactococcus lactis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O20 OCA]. | ||
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| + | ==Reference== | ||
| + | Structure of the transcription regulator CcpA from Lactococcus lactis., Loll B, Kowalczyk M, Alings C, Chieduch A, Bardowski J, Saenger W, Biesiadka J, Acta Crystallogr D Biol Crystallogr. 2007 Apr;63(Pt 4):431-6. Epub 2007, Mar 16. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17372346 17372346] | ||
[[Category: Lactococcus lactis]] | [[Category: Lactococcus lactis]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: Helix-turn-helix]] | [[Category: Helix-turn-helix]] | ||
[[Category: Transcriptional regulator]] | [[Category: Transcriptional regulator]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jun 5 10:08:26 2008'' |
Revision as of 07:08, 5 June 2008
Crystal structure of transcription regulator CcpA of Lactococcus lactis
Overview
Catabolite control protein A (CcpA) functions as master transcriptional regulator of carbon catabolism in Firmicutes. It belongs to the family of bacterial repressor/regulator proteins. Here, the crystal structure of the 76 kDa homodimeric CcpA protein from Lactococcus lactis subsp. lactis IL1403 is presented at 1.9 A resolution in the absence of cognate DNA. The phases were derived by molecular replacement and the structure was refined to crystallographic R and R(free) factors of 0.177 and 0.211, respectively. The presence of a sulfate molecule in the direct vicinity of a putative effector-binding site in the monomer allowed the derivation of a model for the possible binding of small organic effector molecules.
About this Structure
2O20 is a Single protein structure of sequence from Lactococcus lactis. Full crystallographic information is available from OCA.
Reference
Structure of the transcription regulator CcpA from Lactococcus lactis., Loll B, Kowalczyk M, Alings C, Chieduch A, Bardowski J, Saenger W, Biesiadka J, Acta Crystallogr D Biol Crystallogr. 2007 Apr;63(Pt 4):431-6. Epub 2007, Mar 16. PMID:17372346 Page seeded by OCA on Thu Jun 5 10:08:26 2008
